Human P-Cadherin Antibody Summary
Accession # CAA45177
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human P‑Cadherin by Western Blot. Western blot shows lysate of ZR-75 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human P-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF861) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for P-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human P‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of ZR-75 human breast cancer cell line and A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for P-Cadherin at approximately 135 kDa (as indicated) using 10 µg/mL of Goat Anti-Human P-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF861) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Placental (P)-Cadherin (PCAD) is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human P-Cadherin is an 829 amino acid (aa) protein with a 26 aa signal sequence and an 803 aa propeptide. The mature protein begins at aa 108 and has a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human and mouse mature PCAD proteins share 87% homology.
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Citation for Human P-Cadherin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Trophoblast differentiation defect in human embryonic stem cells lacking PIG-A and GPI-anchored cell-surface proteins.
Authors: Chen G, Ye Z, Yu X, Zou J, Mali P, Brodsky RA, Cheng L
Cell Stem Cell, 2008;2(4):345-55.
Sample Types: Whole Cells
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