Human p16INK4a / CDKN2A Antibody

Catalog # Availability Size / Price Qty
AF5779
AF5779-SP
Detection of Human p16INK4a/CDKN2A by Western Blot.
3 Images
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (2)

Human p16INK4a / CDKN2A Antibody Summary

Species Reactivity
Human
Specificity
Detects human p16INK4a/CDKN2A in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human p16INK4a/CDKN2A
Glu2-Asp156
Accession # P42771
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
0.3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human p16INK4a/CDKN2A antibody by Western Blot. View Larger

Detection of Human p16INK4a/CDKN2A by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, and Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human p16INK4a/CDKN2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5779) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for p16INK4a/CDKN2A at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry p16INK4a / CDKN2A antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

p16INK4a / CDKN2A in HeLa Human Cell Line. p16INK4a / CDKN2A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human p16INK4a / CDKN2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5779) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human p16INK4a / CDKN2A antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human p16INK4a / CDKN2A by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for p16INK4a / CDKN2A at approximately 24 kDa (as indicated) using 10 µg/mL of Goat Anti-Human p16INK4a / CDKN2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5779) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p16INK4a / CDKN2A

p16INK4a (16 kDa Inhibitor of CDK4-a; also MTS1, CDK41 and CDKN2) is a 16 kDa member of the CDKN2 cyclin-dependent kinase inhibitor family of molecules. It is widely expressed (although not in skeletal muscle) and serves as a negative regulator of cell proliferation. It does so by associating with CDK4 or 6, thereby blocking cyclin binding and subsequent Ser/Thr kinase activity. Human p16INK4a is 156 amino acids (aa) in length. It contains four “L” shaped ankyrin repeats
(aa 11‑139) that interact with cyclin. There are at least two splice variants for p16INK4a. One is termed p12 and shows a 65 aa substitution for aa 52‑156; the other simply shows an alternate start site at Met52. Full‑length human p16INK4a shares 63% aa identity with mouse p16INK4a.

Long Name
p16 Cyclin Dependent Kinase 4 Inhibitor 2A
Entrez Gene IDs
1029 (Human); 12578 (Mouse); 25163 (Rat)
Alternate Names
ARF; CDK4 inhibitor p16-INK4; CDK4I; CDK4IP14ARF; CDKN2; CDKN2A; cell cycle negative regulator beta; CMM2P16-INK4A; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A; INK4; INK4a; MLM; MLMP16INK4; MTS-1; MTS1P14; Multiple tumor suppressor 1; p14; p14ARF; p16; p16-INK4; p16INK4a; p16-INK4a; P19; p19ARF; TP16

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Citations for Human p16INK4a / CDKN2A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. TRPC3 shapes the ER-mitochondria Ca2+ transfer characterizing tumour-promoting senescence
    Authors: V Farfariell, DV Gordienko, L Mesilmany, Y Touil, E Germain, I Fliniaux, E Desruelles, D Gkika, M Roudbaraki, G Shapovalov, L Noyer, M Lebas, L Allart, N Zienthal-G, O Iamshanova, F Bonardi, M Figeac, W Laine, J Kluza, P Marchetti, B Quesnel, D Metzger, D Bernard, JB Parys, L Lemonnier, N Prevarskay
    Nature Communications, 2022;13(1):956.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. PRMT5 inhibition disrupts splicing and stemness in glioblastoma
    Authors: P Sachamitr, JC Ho, FE Ciamponi, W Ba-Alawi, FJ Coutinho, P Guilhamon, MM Kushida, FMG Cavalli, L Lee, N Rastegar, V Vu, M Sánchez-Os, J Coulombe-H, E Kanshin, H Whetstone, M Durand, P Thibault, K Hart, M Mangos, J Veyhl, W Chen, N Tran, BC Duong, AM Aman, X Che, X Lan, O Whitley, O Zaslaver, D Barsyte-Lo, LM Richards, I Restall, A Caudy, HL Röst, ZQ Bonday, M Bernstein, S Das, MD Cusimano, J Spears, GD Bader, TJ Pugh, M Tyers, M Lupien, B Haibe-Kain, H Artee Luch, S Weiss, KB Massirer, P Prinos, CH Arrowsmith, PB Dirks
    Nature Communications, 2021;12(1):979.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Bacterial genotoxins induce T�cell senescence
    Authors: SL Mathiasen, L Gall-Mas, IS Pateras, SDP Theodorou, MRJ Namini, MB Hansen, OCB Martin, CK Vadivel, K Ntostoglou, D Butter, M Givskov, C Geisler, AN Akbar, VG Gorgoulis, T Frisan, N Ødum, T Krejsgaard
    Cell Reports, 2021;35(10):109220.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule
    Authors: P Vijayaraj, A Minasyan, A Durra, S Karumbayar, M Mehrabi, CJ Aros, SD Ahadome, DW Shia, K Chung, JM Sandlin, KF Darmawan, KV Bhatt, CC Manze, MK Paul, DC Wilkinson, W Yan, AT Clark, TM Rickabaugh, WD Wallace, TG Graeber, R Damoiseaux, BN Gomperts
    Cell Rep, 2019;29(11):3488-3505.e9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Exposure of human melanocytes to UVB twice and subsequent incubation leads to cellular senescence and senescence-associated pigmentation through the prolonged p53 expression
    Authors: SY Choi, BH Bin, W Kim, E Lee, TR Lee, EG Cho
    J. Dermatol. Sci., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. MAX inactivation is an early event in GIST development that regulates p16 and cell proliferation
    Authors: IM Schaefer, Y Wang, CW Liang, N Bahri, A Quattrone, L Doyle, A Mariño-Enr, A Lauria, M Zhu, M Debiec-Ryc, S Grunewald, JF Hechtman, A Dufresne, CR Antonescu, C Beadling, ET Sicinska, M van de Rij, GD Demetri, M Ladanyi, CL Corless, MC Heinrich, CP Raut, S Bauer, JA Fletcher
    Nat Commun, 2017;8(0):14674.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Adiponectin corrects premature cellular senescence and normalizes antimicrobial peptide levels in senescent keratinocytes
    Authors: Taewon Jin
    Biochem Biophys Res Commun, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Induction of heparanase by HPV E6 oncogene in head and neck squamous cell carcinoma.
    Authors: Hirshoren N, Bulvik R, Neuman T, Rubinstein A, Meirovitz A, Elkin M
    J Cell Mol Med, 2014;18(1):181-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Human p16INK4a / CDKN2A Antibody

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Human p16INK4a / CDKN2A Antibody
By Nicole Malet on 08/12/2021
Application: Simple Western Sample Tested: HEK293 human embryonic kidney cell line Species: Human

Human p16INK4a/CDKN2A Antibody
Ref:AF5779 R et D Systems
Efficacité validée dans conditions suivantes:
Dilution anticorps p16= 1/10è (0,2mg/ml solution stock)
[HEK]= 0,65mg/ml
Anticorps 2aire goat prêt à l’emploi


Human p16INK4a / CDKN2A Antibody
By Juliana Redondo on 05/20/2016
Application: WB Sample Tested: Mesenchymal stem cells Species: Human

5% non fat milk as blocking agent for 1 hour and overnight incubation with primary antibody at 1:3000. Specific band at 16kDa as expected, but also band at 25 KDa.
Cells at passage 2 had low p16 expression, while at passage 6 expression was increased as expected. Loading: p2,p6-p2,p6-p2,p6-p2,p6 of MSCs from 4 different individuals.