Human P2X7/P2RX7 Antibody

Catalog # Availability Size / Price Qty
MAB9790-100
MAB9790-SP
Human P2X7/P2RX7 Antibody in Immunocytochemistry
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Human P2X7/P2RX7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human P2X7/P2RX7 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 545230
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human P2X7/P2RX7
Asp356-Tyr595
Accession # Q99572
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry P2X7/P2RX7 in WM‑115 Human Cell Line. View Larger

P2X7/P2RX7 in WM‑115 Human Cell Line. P2X7/P2RX7 was detected in immersion fixed WM-115 human malignant melanoma cell line using Mouse Anti-Human P2X7/P2RX7 Monoclonal Antibody (Catalog # MAB9790) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P2X7/P2RX7

Human P2X purinoceptor 7 (aka P2X7) is a 595 aminoacids (aa) protein encoded by the P2RX7 gene. P2X7 receptors belong to the family of ATP-gated ion channels, and their activity can be found in cells of hemopoietic lineage including macrophages, microglia, and certain lymphocytes. They mediate the influx of Ca2+ and Na+ and the release of pro-inflammatory cytokines. At least 8 different human P2X7 isoforms formed by alternative splicing have been reported, ranging from 128 aa to 595 aa. The P2X7 subunits can form homomeric receptors only with a typical P2X receptor structure.

Long Name
P2X Purinergic Receptor 7
Entrez Gene IDs
5027 (Human); 18439 (Mouse); 29665 (Rat)
Alternate Names
ATP receptor; MGC20089; P2RX7; P2X purinoceptor 7; P2X7; P2X7P2X7 receptor; P2X7R; P2Z receptor; purinergic receptor P2X, ligand-gated ion channel, 7; purinergic receptor P2X7 variant A; Purinergic receptor

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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