Human P2X7/P2RX7 Antibody

Catalog # Availability Size / Price Qty
P2X7/P2RX7 in WM‑115 Human Cell Line.
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Product Details
Supplemental Products

Human P2X7/P2RX7 Antibody Summary

Species Reactivity
Detects human P2X7/P2RX7 in direct ELISAs.
Monoclonal Mouse IgG2A Clone # 545230
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human P2X7/P2RX7
Accession # Q99572
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry P2X7/P2RX7 antibody in WM-115 Human Cell Line by Immunocytochemistry (ICC). View Larger

P2X7/P2RX7 in WM‑115 Human Cell Line. P2X7/P2RX7 was detected in immersion fixed WM-115 human malignant melanoma cell line using Mouse Anti-Human P2X7/P2RX7 Monoclonal Antibody (Catalog # MAB9790) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P2X7/P2RX7

Human P2X purinoceptor 7 (aka P2X7) is a 595 aminoacids (aa) protein encoded by the P2RX7 gene. P2X7 receptors belong to the family of ATP-gated ion channels, and their activity can be found in cells of hemopoietic lineage including macrophages, microglia, and certain lymphocytes. They mediate the influx of Ca2+ and Na+ and the release of pro-inflammatory cytokines. At least 8 different human P2X7 isoforms formed by alternative splicing have been reported, ranging from 128 aa to 595 aa. The P2X7 subunits can form homomeric receptors only with a typical P2X receptor structure.

Long Name
P2X Purinergic Receptor 7
Entrez Gene IDs
5027 (Human); 18439 (Mouse); 29665 (Rat)
Alternate Names
ATP receptor; MGC20089; P2RX7; P2X purinoceptor 7; P2X7; P2X7P2X7 receptor; P2X7R; P2Z receptor; purinergic receptor P2X, ligand-gated ion channel, 7; purinergic receptor P2X7 variant A; Purinergic receptor

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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