Human P2X7/P2RX7 Antibody Summary
Accession # Q99572
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
P2X7/P2RX7 in WM‑115 Human Cell Line. P2X7/P2RX7 was detected in immersion fixed WM-115 human malignant melanoma cell line using Mouse Anti-Human P2X7/P2RX7 Monoclonal Antibody (Catalog # MAB9790) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human P2X purinoceptor 7 (aka P2X7) is a 595 aminoacids (aa) protein encoded by the P2RX7 gene. P2X7 receptors belong to the family of ATP-gated ion channels, and their activity can be found in cells of hemopoietic lineage including macrophages, microglia, and certain lymphocytes. They mediate the influx of Ca2+ and Na+ and the release of pro-inflammatory cytokines. At least 8 different human P2X7 isoforms formed by alternative splicing have been reported, ranging from 128 aa to 595 aa. The P2X7 subunits can form homomeric receptors only with a typical P2X receptor structure.
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Cell and Tissue Staining Kits
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