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Human Pannexin-3 Antibody

Catalog #: MAB8169
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8169
MAB8169-SP

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Detection of Human Pannexin-3 by Western Blot.
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Human Pannexin-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pannexin-3 in ELISA.
Source
Monoclonal Mouse IgG2B Clone # 788905
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Pannexin-3
Tyr289-Thr384
Accession # Q96QZ0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Pannexin-3 antibody by Western Blot. View Larger

Detection of Human Pannexin-3 by Western Blot. Western blot shows lysates of SW1353 human chondrosarcoma cell line and Bowes human melanoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pannexin-3 Monoclonal Antibody (Catalog # MAB8169) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pannexin-3 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pannexin-3

Pannexin-3 (PANX3) is an approximately 45 kDa member of the Pannexin family of four-transmembrane channel proteins with a conserved pattern of cysteines. It is expressed in skin, cochlea, and developing hard tissues including cartilage and bone. In osteoblasts, it forms Ca2+ channels in the endoplasmic reticulum and promotes osteogenic differentiation. It also forms gap junction channels that pass Ca2+ between cells, and ATP-releasing hemichannels in the plasma membrane. Within aa 289-384 (in the C-terminal cytoplasmic domain), human Pannexin-3 shares 95% and 99% aa sequence identity with mouse and rat Pannexin-3, respectively.

Entrez Gene IDs
116337 (Human); 208098 (Mouse); 315567 (Rat)
Alternate Names
gap junction protein pannexin 3; pannexin 3; Pannexin3; Pannexin-3; PANX3; PX3

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Citation for Human Pannexin-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Pannexin-1 in Human Lymphatic Endothelial Cells Regulates Lymphangiogenesis
    Authors: J Boucher, C Simonneau, G Denet, J Clarhaut, AC Balandre, M Mesnil, L Cronier, A Monvoisin
    Int J Mol Sci, 2018-05-24;19(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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