Human PAR1 Antibody

Catalog # Availability Size / Price Qty
Detection of PAR1 in HT‑29 Human Cell Line by Flow Cytometry.
2 Images
Product Details
Citations (2)
Supplemental Products

Human PAR1 Antibody Summary

Species Reactivity
Detects human PAR1 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human PAR1
Arg27-Thr102, Ser375-Thr425
Accession # P25116
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human PAR1
Flow Cytometry
2.5 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PAR1 antibody in HT-29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PAR1 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Human PAR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3855, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunocytochemistry PAR1 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC). View Larger

PAR1 in HT‑29 Human Cell Line. PAR1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Goat Anti-Human PAR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3855) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PAR1

Human Proteinase-Activated Receptor 1 (hPAR1), also known as thrombin receptor, is a 65-70 kDa, 399 amino acid long member of the seven-transmembrane superfamily of cell-surface G protein-coupled receptors. PAR1 is activated by thrombin cleavage of its N-terminal propeptide in the extracellular domain. Human PAR1 is widely expressed in many cell types including endothelial cells, and it has been implicated in a variety of inflammatory responses. Over the regions used as immunogen, human and mouse PAR1 proteins are 58% identical in the region spanning the propeptide and extracellular domains, and 84% identical in the cytoplasmic tail.

Long Name
Protease-Activated Receptor 1
Entrez Gene IDs
2149 (Human); 14062 (Mouse); 25439 (Rat)
Alternate Names
Cf2r; CF2RHTR; coagulation factor II (thrombin) receptor; Coagulation factor II receptor; F2R; HTR; PAR1; PAR1PAR-1protease-activated receptor 1; Protease-Activated Receptor 1; Thrombin receptor; ThrR; TRGPC; TRproteinase-activated receptor 1

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Citations for Human PAR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. An acidic microenvironment sets the humoral pattern recognition molecule PTX3 in a tissue repair mode.
    Authors: Doni A, Musso T, Morone D, Bastone A, Zambelli V, Sironi M, Castagnoli C, Cambieri I, Stravalaci M, Pasqualini F, Laface I, Valentino S, Tartari S, Ponzetta A, Maina V, Barbieri S, Tremoli E, Catapano A, Norata G, Bottazzi B, Garlanda C, Mantovani A
    J Exp Med, 2015;212(6):905-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Oncogenic epidermal growth factor receptor up-regulates multiple elements of the tissue factor signaling pathway in human glioma cells.
    Authors: Magnus N, Garnier D, Rak J
    Blood, 2010;116(5):815-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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