|Detection of Human Pax7 by Western Blot. Western blot shows lysates of human muscle tissue. PVDF Membrane was probed with 0.5 µg/mL of Human Pax7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6670) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pax7 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
PAX7 (Paired box 7A; also HuP1) is a 57 kDa member of the paired homeobox family of transcription factors. It is expressed in select neurons (in rodent) and skeletal muscle satellite cells, where, in the latter, it promotes both a muscle stem cell state (PAX7+ desmin- cells) and a lineage commitment state (PAX7+ desmin+ cells). PAX7 forms heterodimers with PAX3, and interacts with the regulatory regions of the Myf5 locus. Human PAX7(A) is 520 amino acids (aa) in length. It contains an N‑terminal paired box DNA-binding domain (aa 34-163), a homeodomain (aa 218-275) and a C-terminal poly-alanine region (aa 340-346). Multiple splice forms of PAX7 exist. There is a 54 kDa isoform B that contains a 38 aa substitution for aa 468-520, plus a second isoform that shows a deletion of Gly151 and Leu152. There is also a chromosomal rearrangement that fuses aa 30-385 of PAX7 to aa 211-265 of FOXO1A/FKHR. Over aa 1-196, human PAX7 shares 86% and 96% aa identity with human PAX3 and mouse PAX7, respectively.
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