Human Pax7 Antibody

Catalog # Availability Size / Price Qty
AF6670
AF6670-SP
Detection of Human Pax7 by Western Blot.
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Human Pax7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pax7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Pax3 is observed and approximately 4% cross-reactivity with rhPax1 and rhPax6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Pax7
Met1-Gly196
Accession # P23759
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Pax7 by Western Blot. View Larger

Detection of Human Pax7 by Western Blot. Western blot shows lysates of human muscle tissue. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human Pax7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6670) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pax7 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pax7

PAX7 (Paired box 7A; also HuP1) is a 57 kDa member of the paired homeobox family of transcription factors. It is expressed in select neurons (in rodent) and skeletal muscle satellite cells, where, in the latter, it promotes both a muscle stem cell state (PAX7+ desmin- cells) and a lineage commitment state (PAX7+ desmin+ cells). PAX7 forms heterodimers with PAX3, and interacts with the regulatory regions of the Myf5 locus. Human PAX7(A) is 520 amino acids (aa) in length. It contains an N‑terminal paired box DNA-binding domain (aa 34-163), a homeodomain (aa 218-275) and a C-terminal poly-alanine region (aa 340-346). Multiple splice forms of PAX7 exist. There is a 54 kDa isoform B that contains a 38 aa substitution for aa 468-520, plus a second isoform that shows a deletion of Gly151 and Leu152. There is also a chromosomal rearrangement that fuses aa 30-385 of PAX7 to aa 211-265 of FOXO1A/FKHR. Over aa 1-196, human PAX7 shares 86% and 96% aa identity with human PAX3 and mouse PAX7, respectively.

Long Name
Paired Box Gene 7
Entrez Gene IDs
5081 (Human); 18509 (Mouse); 500574 (Rat)
Alternate Names
FLJ37460; HUP1; paired box 7; paired box gene 7; paired box homeotic gene 7; paired box protein Pax-7; paired domain gene 7; PAX7 transcriptional factor; Pax7; PAX7B; RMS2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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