Human PD-L1/B7-H1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB1561R-100
MAB1561R-SP
Human PD-L1/B7-H1 Antibody in Flow Cytometry
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Human PD-L1/B7-H1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PD-L1/B7-H1 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 130021R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PD-L1/B7-H1
Phe19-Thr239
Accession # Q9NZQ7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PD-L1/B7-H1 DuoSet ELISA Kit (Catalog # DY156) for convenient development of a sandwich ELISA or the Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit (Catalog # DB7H10) for a complete optimized ELISA.

 
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of PD-L1/B7-H1  in HEK293 Human Cell Line Transfected with Human PD-L1/B7-H1 and eGFP by Flow  Cytometry. View Larger

Detection of PD-L1/B7-H1 in HEK293 Human Cell Line Transfected with Human PD-L1/B7-H1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) PD-L1/B7-H1 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561R) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.

Human PD-L1/B7-H1 ELISA Standard Curve. Recombinant Human PD-L1/B7-H1 protein was serially diluted 2-fold and captured by Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L1/B7-H1

Human B7 homolog 1 (B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PDL2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. Their extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, there is about 20-25% amino acid identity. Human and mouse B7-H1 share approximately 70% amino acid sequence identity. B7-H1 has been identified as one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The B7-H1 gene encodes a 291 amino acid (aa) type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular domain, a 21 aa transmembrane region, and a 31 aa cytoplasmic domain. Human B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN-gamma stimulation. Interaction of B7-H1 with PD-1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

References
  1. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
  2. Freeman, G.J. et al. (2000) J. Exp. Med. 192:1027.
  3. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
Long Name
Programmed Death Ligand 1
Entrez Gene IDs
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names
B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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