Human PDGF R alpha Antibody

(13 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse PDGF R alpha, recombinant human (rh) PDGF R beta, rhFGF R2, and rhFGF R3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PDGF R alpha
    Gln24-Glu524
    Accession # P16234
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human PDGF R alpha (Catalog # 322-PR)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize PDGF‑AA-induced proliferation in the WS-1 human fetal skin fibroblast cell line. The Neutralization Dose (ND50) is typically 1-6 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody.

Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the WS-1 human fetal skin fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑307‑NA). The ND50 is typically 1-6 µg/mL.

Immunohistochemistry
PDGF R alpha in Human Breast Cancer Tissue.

PDGF R alpha was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 15 µg/mL overnight at
4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry
PDGF R alpha in Human Ovary.

PDGF R alpha was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry
PDGF R alpha in Human Osteosarcoma. PDGF R alpha was detected in immersion fixed paraffin-embedded sections of human osteosarcoma using Goat Anti-Human PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDGF R alpha

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF‑AA, PDGF‑BB, and PDGF‑AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB and -AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell, and liver endothelial cells. Soluble PDGF R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

  • References:
    1. Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
  • Long Name:
    Platelet-derived Growth Factor Receptor alpha
  • Entrez Gene IDs:
    5156 (Human); 18595 (Mouse)
  • Alternate Names:
    alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Adaptive changes of telocytes in the urinary bladder of patients affected by neurogenic detrusor overactivity
    Authors: C Traini, MS Fausssone-, D Guasti, G Del Popolo, J Frizzi, S Serni, MG Vannucchi
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  2. Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia
    Authors: SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro
    PLoS ONE, 2017;12(8):e0182265.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Telocytes in normal and keratoconic human cornea: an immunohistochemical and transmission electron microscopy study
    Authors: M Marini, R Mencucci, I Rosa, E Favuzza, D Guasti, L Ibba-Manne, M Manetti
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  4. Identification and characterization of PDGFRalpha+ mesenchymal progenitors in human skeletal muscle.
    Authors: Uezumi A, Fukada S, Yamamoto N, Ikemoto-Uezumi M, Nakatani M, Morita M, Yamaguchi A, Yamada H, Nishino I, Hamada Y, Tsuchida K
    Cell Death Dis, 2014;5(0):e1186.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Telocytes in Crohn's disease.
    Authors: Milia A, Ruffo M, Manetti M, Rosa I, Conte D, Fazi M, Messerini L, Ibba-Manneschi L
    J Cell Mol Med, 2013;17(12):1525-36.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  6. Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothelial cells.
    Authors: Kusuhara, Sentaro, Fukushima, Yoko, Fukuhara, Shigetom, Jakt, Lars Mar, Okada, Mitsuhir, Shimizu, Yuri, Hata, Masayuki, Nishida, Kohji, Negi, Akira, Hirashima, Masanori, Mochizuki, Naoki, Nishikawa, Shin-Ich, Uemura, Akiyoshi
    PLoS ONE, 2012;7(9):e45858.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. Platelet-derived growth factor-alpha receptor activation is required for human cytomegalovirus infection.
    Authors: Soroceanu L, Akhavan A, Cobbs CS
    Nature, 2008;455(7211):391-5.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  8. Distinctions between fetal and adult human platelet-derived growth factor-responsive neural precursors.
    Authors: Chojnacki A, Kelly JJ, Hader W, Weiss S
    Ann. Neurol., 2008;64(2):127-42.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
    Authors: Dolloff NG, Russell MR, Loizos N, Fatatis A
    Cancer Res., 2007;67(2):555-62.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
    Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
    Blood, 2006;107(5):1896-902.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  11. Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas.
    Authors: Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K
    Cancer Res., 2005;65(16):7241-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: Immunodepletion
  12. Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
    Authors: Nili N, Cheema AN, Giordano FJ, Barolet AW, Babaei S, Hickey R, Eskandarian MR, Smeets M, Butany J, Pasterkamp G, Strauss BH
    Am. J. Pathol., 2003;163(3):869-78.
    Species: Rabbit
    Sample Type: Cell Lysates
    Application: WB
  13. Content and activity of cAMP response element-binding protein regulate platelet-derived growth factor receptor-alpha content in vascular smooth muscles.
    Authors: Watson PA, Vinson C, Nesterova A, Reusch JE
    Endocrinology, 2002;143(8):2922-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedSpinal cord,Brain tissue
SpeciesMouse

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