Human Pepsinogen A5/PGA5 Antibody

Catalog # Availability Size / Price Qty
MAB8457-100
MAB8457-SP
Human Pepsinogen A5/PGA5 Antibody in Western Blot
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Human Pepsinogen A5/PGA5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pepsinogen A5/PGA5 protein in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 974731
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell, HEK293-derived human Pepsinogen A5/PGA5 protein
Ile16-Ala388
Accession # P0DJD9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunohistochemistry
0.5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human Pepsinogen A5/PGA5 by Western Blot. View Larger

Detection of Human Pepsinogen A5/PGA5 by Western Blot. Western blot shows lysates of human stomach cancer tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pepsinogen A5/PGA5 Monoclonal Antibody (Catalog # MAB8457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pepsinogen A5/PGA5 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Pepsinogen  A5/PGA5 in Human Stomach. View Larger

Pepsinogen A5/PGA5 in Human Stomach. Pepsinogen A5/PGA5 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Pepsinogen A5/PGA5 Monoclonal Antibody (Catalog # MAB8457) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection  of Human Pepsinogen A5/PGA5 by Simple  WesternTM. View Larger

Detection of Human Pepsinogen A5/PGA5 by Simple WesternTM. Simple Western lane view shows lysates of human stomach tissue and human stomach cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for Pepsinogen A5/PGA5 at approximately 48 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Pepsinogen A5/PGA5 Monoclonal Antibody (Catalog # MAB8457). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pepsinogen A5/PGA5

Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens which are then converted to active pepsins by the acidic pH of gastric juices (1-3). PGA3, PGA4, and PGA5 are human Pepsinogen A isozymogens that differ in sequence by 2­4 amino acid (aa) residues (4, 5). This recombinant human Pepsinogen A corresponds to PGA5. Human Pepsinogen A isozymogens share approximately 56% aa sequence identity with mouse and rat Pepsinogen A isozymogens. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin (6, 7). Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.

References
  1. Athauda, S.B. et al. (1989) J. Biochem. 106:920.
  2. Kageyama, T. et al. (1989) J. Biochem. 105:15.
  3. Kageyama, T. (2002) Cell. Mol. Life Sci. 59:288.
  4. Zwiers, A. et al. (1994) Clin. Nephrol. 41:153.
  5. Nakai, H. et al. (1986) Cytogenet. Cell Genet. 43:215.
  6. Marciniszyn, J., Jr. et al. (1976) J. Biol. Chem. 251:7088.
  7. Kageyama, T. and K. Takahashi (1980) J. Biochem. 88:571.
Entrez Gene IDs
5222 (Human)
Alternate Names
EC 3.4.23; EC 3.4.23.1; pepsin A; pepsinogen 5, group I (pepsinogen A); Pepsinogen A5; PGA3; PGA4; PGA5

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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