Human Perilipin-2 Antibody

Detection of Human Perilipin-2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and JAR human choriocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB7634) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Perilipin-2 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Perilipin-2 by Simple Western^TM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Perilipin-2 at approximately 58 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB7634). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Detection of Perilipin-2 by Western Blot

ApoE2 and apoE3, but not apoE4, partially rescue alpha -synuclein accumulation in apoE-deficient cerebral organoids. The APOE−/− iPSC-derived cerebral organoids at Day 90 were treated with conditioned media of immortalized astrocytes from APOE2-target replacement (TR), APOE3-TR, or APOE4-TR mice for 5 days. Conditioned media from Apoe-KO astrocytes were used as a control. a, A schematic workflow of the rescue experiments. b–i, Amounts of EEA1 (c), LAMP1 (d), GCase (e), LIMP1 (f), Plin2 (g), alpha Syn (h) and p-alpha Syn (i) in the RIPA fraction of the cerebral organoids after treatments were quantified by Western blotting. j–l, Amounts of total alpha Syn (k) and p-alpha Syn (l) in the SDS fraction of cerebral organoids after treatments were quantified by Western blotting. All data were normalized to beta -actin levels. 3 cerebral organoids were pooled and analyzed as one sample. All data are expressed as mean ± SEM (n = 5 samples/each). Experiments were repeated in three independent differentiation batches. One-way ANOVA was performed to determine statistical significance. **p < 0.01, ****p < 0.0001, n.s., not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34453582), licensed under a CC-BY license. Not internally tested by R&D Systems.