Human Perilipin-2 Antibody

Catalog # Availability Size / Price Qty
MAB7634
MAB7634-SP
Human Perilipin-2 Antibody in Western Blot
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Human Perilipin-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Perilipin-2 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 788620
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Perilipin-2
Met1-His437
Accession # Q99541
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
4 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Perilipin-2 by Western Blot. View Larger

Detection of Human Perilipin-2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and JAR human choriocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB7634) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Perilipin-2 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Perilipin-2 by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Perilipin-2 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Perilipin-2 at approximately 58 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB7634). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Perilipin-2

Perilipin-2 (PLIN2), also known as Adipophilin (ADFP) and Adipose Differentiation-Related Protein (ADRP), is a widely expressed 52 kDa acylated protein that associates with membranes and cytoplasmic lipid droplets. It is upregulated during adipocyte differentiation and is a major component of milk fat globules. Perilipin-2 promotes the cellular uptake and accumulation of long chain fatty acids and regulates the intracellular distribution of phospholipids. It promotes hepatic insulin resistance in response to a high fat diet. Human Perilipin-2 shares 82% and 79% aa sequence identity with mouse and rat Perilipin-2, respectively.

Entrez Gene IDs
123 (Human); 11520 (Mouse); 298199 (Rat)
Alternate Names
ADFP; Adipophilin; Adipose differentiation-related proteinadipophilin; ADRP; MGC10598; perilipin 2; Perilipin2; Perilipin-2; PLIN2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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