Human PERK Antibody

Catalog # Availability Size / Price Qty
AF3999
AF3999-SP
Detection of Human PERK by Western Blot.
3 Images
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)

Human PERK Antibody Summary

Species Reactivity
Human
Specificity
Detects human PERK in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PERK
Ala29-Gln230
Accession # Q9NZJ5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Knockout Validated
PERK is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in PERK knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PERK antibody by Western Blot. View Larger

Detection of Human PERK by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PERK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PERK at approximately 130 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Knockout Validated Western Blot Shows Human PERK Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human PERK Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PERK knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PERK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PERK at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human PERK by Western Blot View Larger

Detection of Human PERK by Western Blot Activation of the ER stress signaling pathway leads to the LX-2 cell apoptosis induced by VP-16.(a) Western blotting analysis of ER stress-associated proteins in LX-2 cells after treatment with VP-16 for 72 h. (b) After exposure to VP-16 for 72 h, the protein phosphorylation levels of PERK and eIF2 alpha were analyzed by western blotting. (c) After exposure to VP-16 for 72 h, the proteins of the IRE1 alpha /ASK1/JNK signaling pathway were analyzed by western blotting. (d,e) LX-2 cells were pretreated with a JNK inhibitor (SP600125, 40 μM) for 1 h, and then treated with 4 μM VP-16 for 72 h. (d) Cell viability was measured using the CCK-8 assay. The percentage of apoptosis cells was analyzed by flow cytometry. The data are presented as the mean ± SD of at least three independent experiments performed in triplicates. **P < 0.01, compared with the group treated with VP-16 alone. (e) Western blotting analysis of the cell lysates was performed using the indicated antibodies. In all of the western blotting analyses, beta -actin was used as a loading control. *P < 0.05, compared with the control group. #P < 0.05, compared with the group treated with VP-16 alone. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27680712), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PERK

PERK, a type 1 ER membrane kinase, mediates eIF2 alpha phosphorylation at Ser51 during the UPR (unfolded protein response). Protein synthesis is inhibited, thereby reducing the burden of protein substrate for the ER folding and degradation mechanism. Phosphorylation of eIF2 alpha also selectively promotes the expression of UPR target genes such as Chop and BiP. PERK may also play a role in tumor cell adaptation to hypoxic stress by regulating the translation of angiogenic factors necessary for the development of functional microvessels. Mutations in PERK are responsible for the rare autosomal-recessive disorder, WRS (Wolcott-Rallison syndrome).

Long Name
Eukaryotic Translation Initiation Factor 4B
Entrez Gene IDs
9451 (Human); 13666 (Mouse); 29702 (Rat)
Alternate Names
DKFZp781H1925; EC 2.7.11.1; EIF2AK3; eukaryotic translation initiation factor 2 alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; hsPEK; PEK; PEKPERKPancreatic eIF2-alpha kinase; PERK; PRKR-like endoplasmic reticulum kinase; WRS

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Citations for Human PERK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Significance of calreticulin as a prognostic factor in endometrial cancer
    Authors: Q Xu, C Chen, G Chen, W Chen, D Zhou, Y Xie
    Oncol Lett, 2018-04-13;15(6):8999-9008.
  2. Belantamab Mafodotin (GSK2857916) Drives Immunogenic Cell Death and Immune-mediated Antitumor Responses In Vivo
    Authors: Montes de Oca R, Alavi AS, Vitali N et al.
    Molecular Cancer Therapeutics
  3. Etoposide Induces Apoptosis in Activated Human Hepatic Stellate Cells via ER Stress
    Authors: Chen Wang, Feng Zhang, Yu Cao, Mingming Zhang, Aixiu Wang, Mingcui Xu et al.
    Scientific Reports
  4. Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation.
    Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H
    PLoS ONE, 2015-03-17;10(3):e0119738.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Characterization of a novel PERK kinase inhibitor with antitumor and antiangiogenic activity.
    Authors: Atkins C, Liu Q, Minthorn E, Zhang S, Figueroa D, Moss K, Stanley T, Sanders B, Goetz A, Gaul N, Choudhry A, Alsaid H, Jucker B, Axten J, Kumar R
    Cancer Res, 2013-01-18;73(6):1993-2002.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Polycystin-2 down-regulates cell proliferation via promoting PERK-dependent phosphorylation of eIF2alpha.
    Authors: Liang G, Yang J, Wang Z
    Hum. Mol. Genet., 2008-07-29;17(20):3254-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. PERK-mediated expression of peptidylglycine alpha -amidating monooxygenase supports angiogenesis in glioblastoma
    Authors: Himanshu Soni, Julia Bode, Chi D. L. Nguyen, Laura Puccio, Michelle Ne beta ling, Rosario M. Piro et al.
    Oncogenesis

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Human PERK Antibody
By Dharmaraja Allimuthu on 12/27/2017
Application: WB Sample Tested: Pancreatic cancer cells Species: Human