Human PERK Antibody
Human PERK Antibody Summary
Ala29-Gln230
Accession # Q9NZJ5
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human PERK by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PERK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PERK at approximately 130 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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Western Blot Shows Human PERK Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PERK knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PERK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PERK at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PERK
PERK, a type 1 ER membrane kinase, mediates eIF2 alpha phosphorylation at Ser51 during the UPR (unfolded protein response). Protein synthesis is inhibited, thereby reducing the burden of protein substrate for the ER folding and degradation mechanism. Phosphorylation of eIF2 alpha also selectively promotes the expression of UPR target genes such as Chop and BiP. PERK may also play a role in tumor cell adaptation to hypoxic stress by regulating the translation of angiogenic factors necessary for the development of functional microvessels. Mutations in PERK are responsible for the rare autosomal-recessive disorder, WRS (Wolcott-Rallison syndrome).
Product Datasheets
Citations for Human PERK Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation.
Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H
PLoS ONE, 2015;10(3):e0119738.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Characterization of a novel PERK kinase inhibitor with antitumor and antiangiogenic activity.
Authors: Atkins C, Liu Q, Minthorn E, Zhang S, Figueroa D, Moss K, Stanley T, Sanders B, Goetz A, Gaul N, Choudhry A, Alsaid H, Jucker B, Axten J, Kumar R
Cancer Res, 2013;73(6):1993-2002.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Polycystin-2 down-regulates cell proliferation via promoting PERK-dependent phosphorylation of eIF2alpha.
Authors: Liang G, Yang J, Wang Z
Hum. Mol. Genet., 2008;17(20):3254-62.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Average Rating: 5 (Based on 1 Review)
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