Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 566401
View all Peroxiredoxin 4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Peroxiredoxin 4
Met1-Asn271
Accession # Q13162

Specificity

Detects human Peroxiredoxin 4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Peroxiredoxin 4 Antibody

Detection of Human Peroxiredoxin 4 antibody by Western Blot.

Detection of Human Peroxiredoxin 4 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Peroxiredoxin 4 Monoclonal Antibody (Catalog # MAB5460) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Peroxiredoxin 4 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Peroxiredoxin 4 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Peroxiredoxin 4 in HeLa Human Cell Line.

Peroxiredoxin 4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Peroxiredoxin 4 Monoclonal Antibody (Catalog # MAB5460) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Peroxiredoxin 4 antibody in Human Liver Cancer Tissue by Immunohistochemistry (IHC-P).

Peroxiredoxin 4 in Human Liver Cancer Tissue.

Peroxiredoxin 4 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human Peroxiredoxin 4 Monoclonal Antibody (Catalog # MAB5460) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Peroxiredoxin 4 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

5-25 µg/mL
Sample:   Immersion fixed paraffin-embedded sections of human liver cancer tissue

Western Blot

1 µg/mL
Sample:

HEK293 human embryonic kidney cell line and K562 human chronic myelogenous leukemia cell line

Reviewed Applications

Read 1 review rated 5 using MAB5460 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Peroxiredoxin 4

Peroxiredoxin 4 (Prx-IV; also known as PRDX4 and thioredoxin peroxidase AOE372) is a 30-32 kDa, widely expressed cytoplasmic antioxidant enzyme that belongs to the typical 2-Cys class of the TSA/ahpC family of peroxiredoxins. It exists as either a homodimer, or heterodimer with PRDX1. PRDX4 reportedly counteracts p53‑mediated generation of ROS, and regulates NF kappa B via I kappa B-alpha. Human PRDX4 is 271 amino acids (aa) in length and contains an N-terminal poly-Leu region (aa 20‑30) and a 159 thioredoxin domain (aa 79‑237). There is one catalytic cysteine at Cys124. Over amino acids 1‑271, human PRDX4 shares 89% aa identity with mouse and rat PRDX4.

Alternate Names

AOE372, PRDX4, Prx4

Entrez Gene IDs

10549 (Human); 53381 (Mouse); 85274 (Rat)

Gene Symbol

PRDX4

UniProt

Q13162 (Human)

Additional Peroxiredoxin 4 Products

Product Documents for Human Peroxiredoxin 4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Peroxiredoxin 4 Antibody

For research use only

Related Research Areas

Oxidative Stress
Redox Enzymes

Customer Reviews for Human Peroxiredoxin 4 Antibody (1)

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  • Human Peroxiredoxin 4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Ovary tissue
    Species: Human
    Verified Customer | Posted 12/01/2021
    Human Peroxiredoxin 4 Antibody MAB5460

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Protocols

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