Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # 702710R
View all CREB Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human CREB S133 site 

Specificity

Detects human CREB when phosphorylated at S133.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Phospho-CREB (S133) Antibody

Detection of Human Phospho-CREB (S133) antibody by Western Blot.

Detection of Human Phospho-CREB (S133) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-CREB (S133) Monoclonal Antibody (Catalog # MAB6906R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-CREB (S133) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-CREB (S133) Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Hela human cervical epithelial carcinoma cells and HEK293 human embryonic kidney cells stimulated with ultraviolet light (UV)

Reviewed Applications

Read 1 review rated 3 using MAB6906R in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CREB

The cAMP response element binding protein (CREB) belongs to the bZIP superfamily of transcription factors, containing a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. When phosphorylated at Ser133, CREB also binds the coactivator CREB binding protein (CBP), which enhances transcription by acetylating histones to facilitate chromatin unraveling.

Long Name

cAMP Response Element-binding Protein

Alternate Names

CREB1

Entrez Gene IDs

1385 (Human); 12912 (Mouse)

Gene Symbol

CREB1

Additional CREB Products

Product Documents for Human Phospho-CREB (S133) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-CREB (S133) Antibody

For research use only

Customer Reviews for Human Phospho-CREB (S133) Antibody (1)

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  • Human Phospho-CREB (S133) Antibody
    Name: Irini Dijkhoff
    Application: Simple Western
    Sample Tested: NHEK human normal epidermal keratinocytes
    Species: Human
    Verified Customer | Posted 05/24/2019
    Protein concentration of 750 ug/mL. Antibody dilution of 1:20. Detection is chemiluminescence.
    Standard JESS run settings.
    Human Phospho-CREB (S133) Antibody MAB6906R

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