Human Phospho-CrkL (Y207) Antibody

Catalog # Availability Size / Price Qty
Detection of Human Phospho-CrkL (Y207) by Western Blot.
1 Image
Product Details
Supplemental Products

Human Phospho-CrkL (Y207) Antibody Summary

Species Reactivity
Detects human Phospho-CrkL (Y207) in direct ELISAs and Western blots.
Monoclonal Mouse IgG2B Clone # 668915
Protein A or G purified from hybridoma culture supernatant
Phosphopeptide containing the human CrkL Y207 site
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Complete Your Experiment


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-CrkL (Y207) antibody by Western Blot. View Larger

Detection of Human Phospho-CrkL (Y207) by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line untreated (-) or treated (+) with 300 U/mL CIP for 60 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-CrkL (Y207) Monoclonal Antibody (Catalog # MAB6910) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-CrkL (Y207) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CrkL

CrkL is a widely-expressed 39 kDa adaptor protein that belongs to the Crk protein family and is abundantly expressed in cells of hematopoietic origin. Through its SH2 and SH3 domains, CrkL initiates interactions with numerous downstream proteins such as CBL, p130Cas, Paxillin, C3G, SOS, PI3K, c-Abl and BCR/Abl. CrkL is involved in such processes as adhesion, migration and the immune response. Furthermore, this adaptor protein plays an essential role in Chronic Myelogenous Leukemia (CML) and is constitutively phosphorylated at Y207 in CML neutrophils by the BCR/Abl kinase.

Long Name
V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein
Entrez Gene IDs
1399 (Human); 12929 (Mouse); 287942 (Rat)
Alternate Names
CrkL; crk-like protein; pCRKL; v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog (avian)-like

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Phospho-CrkL (Y207) Antibody

There are currently no reviews for this product. Be the first to review Human Phospho-CrkL (Y207) Antibody and earn rewards!

Have you used Human Phospho-CrkL (Y207) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review