Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1240C
View all EGFR Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human EGFR  Y1086 site
Accession # P00533

Specificity

Detects human EGFR when phosphorylated at Y1086 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-EGFR (Y1086) Antibody

Detection of Human Phospho-EGFR (Y1086) antibody by Western Blot.

Detection of Human Phospho-EGFR (Y1086) by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1086) Monoclonal Antibody (Catalog # MAB8967) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-EGFR (Y1086) at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-EGFR (Y1086) antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

Phospho-EGFR (Y1086) in A431 Human Cell Line.

EGFR phosphorylated at Y1086 was detected in immersion fixed A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG) using Rabbit Anti-Human Phospho-EGFR (Y1086) Monoclonal Antibody (Catalog # MAB8967) at a 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Phospho-EGFR (Y1086) antibody by Simple WesternTM.

Detection of Human Phospho-EGFR (Y1086) by Simple WesternTM.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-EGFR (Y1086) at approximately 199 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1086) Monoclonal Antibody (Catalog # MAB8967). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Phospho-EGFR (Y1086) Antibody

Application
Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG)

Simple Western

1 µg/mL
Sample: A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG)

Western Blot

0.1 µg/mL
Sample: A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGFR

Epidermal growth factor receptor (EGFR, also known as ErbB1 and HER1) is the founding member of the ErbB family of receptor tyrosine kinases. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation of Y1086 creates a binding site for the adaptor protein GRB2, leading to attenuation of receptor signaling.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

UniProt

P00533

Additional EGFR Products

Product Documents for Human Phospho-EGFR (Y1086) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-EGFR (Y1086) Antibody

For research use only

Citations for Human Phospho-EGFR (Y1086) Antibody

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Protocols

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