Human Phospho-p53 (S46) Antibody

Catalog # Availability Size / Price Qty
AF1489
AF1489-SP
p53 in Human Breast Cancer Tissue.
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Product Details
Citations (1)
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Human Phospho-p53 (S46) Antibody Summary

Species Reactivity
Human
Specificity
Detects human p53 when phosphorylated at S46.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human p53 S46 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry p53 in Human Breast Cancer Tissue. View Larger

p53 in Human Breast Cancer Tissue.

p53 phosphorylated at S46 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human Phospho-p53 (S46) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1489) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human Phospho-p53 (S46) by Western Blot. View Larger

Detection of Human Phospho-p53 (S46) by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 5 hours. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-p53 (S46) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S46) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 30 minutes. For additional reference the membrane was stripped and reprobed for total p53 using Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry p53 in Human Breast. View Larger

p53 in Human Breast.

p53 phosphorylated at S46 was detected in immersion fixed paraffin-embedded sections of human breast array using Rabbit Anti-Human Phospho-p53 (S46) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1489) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Phospho-p53 (S46) by Simple WesternTM. View Larger

Detection of Human Phospho-p53 (S46) by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 5 hours, loaded at 0.2 mg/mL. A specific band was detected for Phospho-p53 (S46) at approximately 65 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-p53 (S46) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1489). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p53

The p53 tumor suppressor protein signals apoptosis in cells that have incurred irreparable genotoxic damage. Phosphorylation of p53 at serine 46 is a critical event leading to the cellular decision to undergo apoptosis rather than cell cycle arrest. Serine 46 can be phosphorylated by either the p38 MAP kinase or the homeodomain-interacting protein kinase 2 (HIPK2).

Entrez Gene IDs
7157 (Human); 22059 (Mouse); 24842 (Rat)
Alternate Names
Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53

Product Datasheets

Citation for Human Phospho-p53 (S46) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. GRP-78 secreted by tumor cells blocks the antiangiogenic activity of bortezomib.
    Authors: Kern J, Untergasser G, Zenzmaier C, Sarg B, Gastl G, Gunsilius E, Steurer M
    Blood, 2009;114(18):3960-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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