Human Phospho-PRAS40 (T246) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB68901
MAB68901-SP
Detection of Human and Mouse Phospho-PRAS40 (T246) by Western Blot.
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Human Phospho-PRAS40 (T246) Antibody Summary

Species Reactivity
Human
Specificity
Detects human and mouse PRAS40 when phosphorylated at T246.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1211A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing the human PRAS40 T246 site
Accession # Q96B36
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse Phospho-PRAS40 (T246) by Western Blot. View Larger

Detection of Human and Mouse Phospho-PRAS40 (T246) by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line and NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/ml Recombinant Human IGF‑I (Catalog # 291-G1) for 60 minutes and 100 ng/ml Recombinant Human PDGF‑BB (Catalog # 220-BB) for 20 minutes. PVDF membrane was probed with 1:1000 µg/mL of Rabbit Anti-Human Phospho-PRAS40 (T246) Monoclonal Antibody (Catalog # MAB68901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PRAS40 (T246) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: PRAS40

PRAS40 (Proline-rich AKT1 substrate 1), also known as Akt1S1 and p39, is a 40-42 kDa cytoplasmic phosphoprotein that lacks generally recognized structural motifs. It is widely expressed and is considered to be key regulator of mTORC1 (mTOR plus Raptor and G beta L), a complex through which Akt signals into the cell. Through phosphorylation, mTORC1 activity is upregulated by PRAS40. In particular, nonphosphorylated PRAS40 binds to and serves as a negative regulator of mTORC1 activity. Upon Insulin signaling, PRAS40 is phosphorylated on Thr246, Ser221 and Ser183. This causes it to bind 14-3-3 and results in its dissociation from mTORC1, freeing up mTOR to regulate (positively or negatively) protein synthesis. Human PRAS40 is 256 amino acids (aa) in length. It contains one extended Pro-rich region (aa 35-96) plus at least nine utilized Ser/Thr phosphorylation sites. There is one alternative start site at Met131. Over aa 119-256, human PRAS40 shares 93% aa sequence identity with mouse PRAS40.

Long Name
40 kDa Proline-rich Akt1 Substrate
Entrez Gene IDs
84335 (Human); 67605 (Mouse); 292887 (Rat)
Alternate Names
40 kDa; AKT1 substrate 1 (proline-rich); AKT1S1; Lobe; MGC2865; PRAS40; proline-rich AKT1 substrate 1,40 kDa proline-rich AKT substrate

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