Human Phospho-STAT1 (Y701) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB2894
MAB2894-SP
Detection of Human Phospho-STAT1 (Y701) by Western Blot.
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Human Phospho-STAT1 (Y701) Antibody Summary

Species Reactivity
Human
Specificity
Detects human STAT1 when phosphorylated at Y701 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1086B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing the human STAT1 Y701 site
Accession # NP009330
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-STAT1 (Y701) by Western Blot. View Larger

Detection of Human Phospho-STAT1 (Y701) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN‑ alpha A (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Phospho-STAT1 (Y701) Monoclonal Antibody (Catalog # MAB2894) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
  • 12 months from date of receipt, -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 °C under sterile conditions after opening.

Background: STAT1

STAT1 (signal transducer and activator of transcription #1) is an 88 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. All STATs contain an N-terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT1 is phosphorylated at Y701 by receptor-associated Janus kinases (JAKs) leading to STAT1 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Long Name
Signal Transducer and Activator of Transcription 1
Entrez Gene IDs
6772 (Human); 20846 (Mouse)
Alternate Names
CANDF7; DKFZp686B04100; ISGF-3; ISGF-3,91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT1; STAT91

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