Human PKC zeta Antibody (453513)

R&D Systems | Catalog # MAB6666

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 453513
View all PKC zeta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKC zeta
Ile454-Val592
Accession # Q05513

Specificity

Detects human PKC zeta in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) PKC iota and rhPKC alpha and no cross-reactivity with rhPKC epsilon, eta, beta 1, beta 2, delta, gamma, nu, mu, or theta is observed. In Western blots, 100% cross-reactivity with recombinant human (rh) PKC iota and no cross-reactivity with rhPKC epsilon or eta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PKC zeta Antibody (453513)

Detection of Human PKC? antibody by Western Blot.

Detection of Human PKC zeta by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PKC zeta Monoclonal Antibody (Catalog # MAB6666) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKC zeta at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PKC zeta Antibody (453513)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 4 reviews rated 4.3 using MAB6666 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PKC zeta

PKC zeta (protein kinase C-zeta, EC=2.7.11.13), also called PKC2, is a 592 amino acid (aa), 79 kDa member of the atypical PKC (aPKC) subfamily of Ser/Thr protein kinases. aPKC proteins can be activated by phorbol esters, but are insensitive to calcium and diacylglycerol. PKC zeta regulates cell polarity in early development, and is necessary for cytokine-induced chemotaxis of macrophages and cancer cells, chemokine-induced adhesion of neutrophils, and polarization of Th2 cells. It is activated by PDK1 phosphorylation, binds ceramide, and translocates to the plasma membrane where it regulates adherens and tight junction formation. PKC zeta contains an OPR domain (aa 15-98), a zinc finger region (aa 130-180), and a protein kinase domain (aa 252-518). PKC zeta potential variants of 409 or 539 aa have alternate start sites at aa 184 or aa 9 (also missing aa 186-233), respectively. Within the region used as an immunogen, human PKC zeta shares 98% aa identity with mouse and rat PKC zeta.

Long Name

Protein Kinase C zeta

Alternate Names

PKC2, PRKCZ

Entrez Gene IDs

5590 (Human); 18762 (Mouse); 25522 (Rat)

Gene Symbol

PRKCZ

UniProt

Q05513

Additional PKC zeta Products

Product Documents for Human PKC zeta Antibody (453513)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PKC zeta Antibody (453513)

For research use only

Customer Reviews for Human PKC zeta Antibody (453513) (4)

4.3 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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3 Stars
25%
2 Stars
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1 Stars
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Showing  1 - 4 of 4 reviews Showing All
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  • Human PKC zeta Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: A549 human lung carcinoma cell line and H69AR human small cell lung cancer cell line
    Species: Human
    Verified Customer | Posted 04/04/2020
    Human PKC zeta Antibody (453513) MAB6666
  • Human PKC zeta Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human PKC zeta Antibody (453513) MAB6666
  • Human PKC zeta Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/08/2018
  • Human PKC zeta Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 10/28/2018
    Human PKC zeta Antibody (453513) MAB6666

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