Human PLA2G2A Antibody

Catalog # Availability Size / Price Qty
Detection of Human PLA2G2A by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Human PLA2G2A Antibody Summary

Species Reactivity
Detects human PLA2G2A in direct ELISAs and Western blots.  In direct ELISAs, no cross-reactivity with recombinant human PLA2G1B, G2A, or G7 is observed.
Monoclonal Mouse IgG2B Clone # 620501
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PLA2G2A
Accession # P14555
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Complete Your Experiment


Recommended Concentration
Western Blot
2 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human PLA2G2A (Catalog # 4925-PL), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PLA2G2A antibody by Western Blot. View Larger

Detection of Human PLA2G2A by Western Blot. Western blot shows lysates of human spleen tissue. PVDF Membrane was probed with 2 µg/mL of Human PLA2G2A Monoclonal Antibody (Catalog # MAB5374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLA2G2A at approximately 17 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLA2G2A

Phospholipase A2 Group IIA (PLA2G2A) is a 17 kDa member of the phospholipase A2 family of enzymes that hydrolyzes the sn-2 ester bond of phospholipids. Human PLA2G2A is synthesized as a 144 amino acid (aa) precursor that contains a 20 aa signal sequence and a 124 aa mature chain. Mature human PLA2G2A shares 72%, 68%, and 60% aa sequence identity with mature rat, mouse, and bovine PLA2G2A, respectively. PLA2G2A is a calcium dependent phospholipase expressed in many cell types associated with inflammation, including platelets, neutrophils, and mast cells. Most secretory PLA2s are stored in cytoplasmic granules and are released into the extracellular environment on appropriate cell activation. Thus, they are present at higher concentrations in the plasma and biologic fluids of patients with systemic inflammatory, autoimmune, or allergic diseases.

Long Name
Phospholipase A2 Group IIA
Entrez Gene IDs
5320 (Human); 18780 (Mouse); 29692 (Rat)
Alternate Names
EC; GIIC sPLA2; Group IIA phospholipase A2; Mom1; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2, group IIA (platelets, synovial fluid); phospholipase A2, membrane associated; PLA2B; PLA2BPLA2; PLA2G2A; PLA2L; PLA2LPLA2S; PLAS1; RASF-A; sPLA2

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Citation for Human PLA2G2A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cellular census of human fibrosis defines functionally distinct stromal cell types and states
    Authors: TB Layton, L Williams, F McCann, M Zhang, M Fritzsche, H Colin-York, M Cabrita, MTH Ng, M Feldmann, SN Sansom, D Furniss, W Xie, J Nanchahal
    Nat Commun, 2020;11(1):2768.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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