Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

CyTof

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 620501
View all PLA2G2A Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PLA2G2A
Asn21-Cys144
Accession # P14555

Specificity

Detects human PLA2G2A in direct ELISAs and Western blots.  In direct ELISAs, no cross-reactivity with recombinant human PLA2G1B, G2A, or G7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PLA2G2A Antibody

Detection of Human PLA2G2A antibody by Western Blot.

Detection of Human PLA2G2A by Western Blot.

Western blot shows lysates of human spleen tissue. PVDF Membrane was probed with 2 µg/mL of Human PLA2G2A Monoclonal Antibody (Catalog # MAB5374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLA2G2A at approximately 17 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PLA2G2A Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human PLA2G2A (Catalog # 4925-PL), see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Human spleen tissue under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PLA2G2A

Phospholipase A2 Group IIA (PLA2G2A) is a 17 kDa member of the phospholipase A2 family of enzymes that hydrolyzes the sn-2 ester bond of phospholipids. Human PLA2G2A is synthesized as a 144 amino acid (aa) precursor that contains a 20 aa signal sequence and a 124 aa mature chain. Mature human PLA2G2A shares 72%, 68%, and 60% aa sequence identity with mature rat, mouse, and bovine PLA2G2A, respectively. PLA2G2A is a calcium dependent phospholipase expressed in many cell types associated with inflammation, including platelets, neutrophils, and mast cells. Most secretory PLA2s are stored in cytoplasmic granules and are released into the extracellular environment on appropriate cell activation. Thus, they are present at higher concentrations in the plasma and biologic fluids of patients with systemic inflammatory, autoimmune, or allergic diseases.

Long Name

Phospholipase A2 Group IIA

Alternate Names

Mom1, PLA2B, PLA2L, RASF-A

Entrez Gene IDs

5320 (Human); 18780 (Mouse); 29692 (Rat)

Gene Symbol

PLA2G2A

UniProt

P14555

Additional PLA2G2A Products

Product Documents for Human PLA2G2A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PLA2G2A Antibody

For research use only

Citations for Human PLA2G2A Antibody

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