Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 736811
View all Plasma Kallikrein/KLKB1 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human KLK-B1
Gly20-Ala638
Accession # P03952

Specificity

Detects human KLK-B1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Plasma Kallikrein/KLKB1 Antibody

Plasma Kallikrein/KLKB1 antibody in Human Liver by Immunohistochemistry (IHC-P).

Plasma Kallikrein/KLKB1 in Human Liver.

Plasma Kallikrein/KLKB1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Plasma Kallikrein/KLKB1 Monoclonal Antibody (Catalog # MAB2497) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Plasma Kallikrein/KLKB1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Reviewed Applications

Read 1 review rated 5 using MAB2497 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Plasma Kallikrein/KLKB1

Human Plasma Kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase. Thus, it plays an important role in blood pressure regulation, fibrinolysis, and neutrophil activation (1). Human Plasma Kallikrein precursor contains a signal peptide (residues 1 to 19) and a pro form sequence (residues 20 to 638). Upon activation, the pro form is converted to a heavy chain and a light chain, which is linked by disulfide bonds and the latter contains the catalytic domain (2). The human Plasma Kallikrein pro form was expressed in the NS0 cells with a foreign signal peptide. The purified enzyme is mainly the pro form. When activated by thermolysin, it displays activity against a fluorogenic peptide substrate as described in Activity Assay Protocol. This activity can be inhibited by Human Serpin G1/C1 Inhibitor (Catalog # http://www.rndsystems.com/product_results.aspx?k=2488-PI">2488-PI).

References

  1. Coleman, R. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. (eds.) p. 1644, Academic Press, San Diego.
  2. Chung, N.W. et al. (1986) Biochemistry 25:2410.

Alternate Names

KLKB1, Plasma Prekallikrein

Entrez Gene IDs

3818 (Human); 16621 (Mouse)

Gene Symbol

KLKB1

UniProt

P03952

Additional Plasma Kallikrein/KLKB1 Products

Product Documents for Human Plasma Kallikrein/KLKB1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Plasma Kallikrein/KLKB1 Antibody

For research use only

Customer Reviews for Human Plasma Kallikrein/KLKB1 Antibody (1)

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  • Human Plasma Kallikrein/KLKB1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver tissue
    Species: Human
    Verified Customer | Posted 01/31/2022
    Human Plasma Kallikrein/KLKB1 Antibody MAB2497

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Protocols

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail