Human PLUNC Antibody (251512)

R&D Systems | Catalog # MAB1897

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Porcine, Chinchilla

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 251512
View all PLUNC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PLUNC
Gln20-Val256
Accession # Q9NP55

Specificity

Detects human PLUNC in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PLUNC Antibody (251512)

Detection of PLUNC by Western Blot

Detection of PLUNC by Western Blot

JNK/c-Jun pathway is involved in LPS-mediated up-regulation of BPIFA1 expression in nasal epithelial cells.(A) Cells were pretreated for 1 h with 20 μM PD98059 (ERK inhibitor), 10 μM SP600125 (JNK inhibitor), or 20 μM SB203580 (p38 inhibitor) and incubated with 10 μg/ml LPS for 2 h. (B) Cells were transfected with a JNK-dominant negative (DN-JNK) mutant for 24 h or pretreated with SP600125 for 30 min prior to incubation with LPS for 1 h. The protein expression levels were determined using western blot. beta -actin was used as the loading control. The western blots were carried out independently in triplicate and results were representative of one of three independent experiments. The expression level of each protein was quantified by signal intensity and was indicated at the bottom of each lane. The quantitative analysis of western blot for three independent experiments was shown in S2 Fig. ANOVA with Tukey’s test was used to compare the overall difference between the groups. P < 0.05 was considered statistically significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26646664), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of PLUNC by Western Blot

Detection of PLUNC by Western Blot

AP-1 is involved in LPS-induced BPIFA1 expression. Cells were pretreated with 10 μM curcumin or tanshinone (inhibitors of c-Jun) for 30 min, followed by incubation with LPS (10 μg/ml) for 2 h. Protein expression levels were determined using western blot and normalized to those of beta -actin. The expression level of each protein is quantified by signal intensity and is indicated at the bottom of each lane. The quantitative analysis of western blot for three independent experiments was shown in S3 Fig. ANOVA with Tukey’s test was used to compare the overall difference between the groups. P < 0.05 was considered statistically significant. (B) Cells were transfected with AP-1-Luc reporter and incubated with LPS (10 μg/ml) for another 2 h. Cell lysates were subjected to luciferase activity assays to determine AP-1 luciferase activity. The results represented mean and standard deviation values from three independent experiments. *, P < 0.05 compared between two groups. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26646664), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of PLUNC by Western Blot

Detection of PLUNC by Western Blot

JNK/c-Jun pathway is involved in LPS-mediated up-regulation of BPIFA1 expression in nasal epithelial cells.(A) Cells were pretreated for 1 h with 20 μM PD98059 (ERK inhibitor), 10 μM SP600125 (JNK inhibitor), or 20 μM SB203580 (p38 inhibitor) and incubated with 10 μg/ml LPS for 2 h. (B) Cells were transfected with a JNK-dominant negative (DN-JNK) mutant for 24 h or pretreated with SP600125 for 30 min prior to incubation with LPS for 1 h. The protein expression levels were determined using western blot. beta -actin was used as the loading control. The western blots were carried out independently in triplicate and results were representative of one of three independent experiments. The expression level of each protein was quantified by signal intensity and was indicated at the bottom of each lane. The quantitative analysis of western blot for three independent experiments was shown in S2 Fig. ANOVA with Tukey’s test was used to compare the overall difference between the groups. P < 0.05 was considered statistically significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26646664), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human PLUNC Antibody (251512)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human PLUNC

Reviewed Applications

Read 1 review rated 5 using MAB1897 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PLUNC

PLUNC, also named SPLUNC1, is a secreted protein that is expressed in the secretory ducts and submucosal glands of tracheobronchial tissues. It is structurally related to bactericidal/permeability-increasing protein (BPI) and lipopolysaccaride binding protein (LBP), which are central to the host defense against gram-negative bacteria. PLUNC belongs to the short subfamily of PLUNC family proteins and has homology only to the N-terminal domains of BPI. PLUNC may function in the innate immune response against bacteria.

Long Name

Palate, Lung, and Nasal Epithelium Associated

Alternate Names

BPIFA1, LUNX, SPLUNC1

Entrez Gene IDs

51297 (Human)

Gene Symbol

BPIFA1

UniProt

Q9NP55

Additional PLUNC Products

Product Documents for Human PLUNC Antibody (251512)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PLUNC Antibody (251512)

For research use only

Citations for Human PLUNC Antibody (251512)

Customer Reviews for Human PLUNC Antibody (251512) (1)

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  • Human PLUNC Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Salivary samples
    Species: Human
    Verified Customer | Posted 08/06/2021
    Human PLUNC Antibody (251512) MAB1897

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