Human Podocalyxin Antibody

Catalog # Availability Size / Price Qty
AF1658
AF1658-SP
Human Podocalyxin Antibody in Western Blot
3 Images
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews

Human Podocalyxin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse Podocalyxin and recombinant human Endoglycan.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Podocalyxin
Ser23-Arg427
Accession # AAB61574
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
BG01V human embryonic stem cells
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Knockout Validated
Podocalyxin/PODXL is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Podocalyxin/PODXL knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Podocalyxin by Western Blot. View Larger

Detection of Human Podocalyxin by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Podocalyxin in Human Kidney. View Larger

Podocalyxin in Human Kidney. Podocalyxin was detected in immersion fixed paraffin-embedded sections of human kidney using 5 µg/mL Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Knockout Validated Western Blot Shows Human Podocalyxin Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Podocalyxin Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Podocalyxin/PODXL knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Podocalyxin at approximately 160 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References
  1.  Li, J. et al. (2001) DNA Seq. 12:407.
  2.  Hara, T. et al. (1999) Immunity 11:567.
Entrez Gene IDs
5420 (Human); 27205 (Mouse)
Alternate Names
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

Product Datasheets

Citations for Human Podocalyxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Organoid single cell profiling identifies a transcriptional signature of glomerular disease
    Authors: JL Harder, R Menon, EA Otto, J Zhou, S Eddy, NL Wys, C O'Connor, J Luo, V Nair, C Cebrian, JR Spence, M Bitzer, OG Troyanskay, JB Hodgin, RC Wiggins, BS Freedman, M Kretzler,,
    JCI Insight, 2019;4(1):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Single-cell transcriptomics reveals gene expression dynamics of human fetal kidney development
    Authors: M Hochane, PR van den Be, X Fan, N Bérenger-C, E Adegeest, M Bialecka, M Nieveen, M Menschaart, SM Chuva de S, S Semrau
    PLoS Biol., 2019;17(2):e3000152.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Overexpression of preeclampsia induced microRNA-26a-5p leads to proteinuria in zebrafish
    Authors: J Müller-Dei, P Schröder, L Beverly-St, R Hiss, J Fiedler, J Nyström, T Thum, H Haller, M Schiffer
    Sci Rep, 2018;8(1):3621.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray
    Authors: S Saito, K Hiemori, K Kiyoi, H Tateno
    Sci Rep, 2018;8(1):3997.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  5. Anti-podocalyxin antibody exerts antitumor effects via antibody-dependent cellular cytotoxicity in mouse xenograft models of oral squamous cell carcinoma
    Authors: S Itai, T Ohishi, MK Kaneko, S Yamada, S Abe, T Nakamura, M Yanaka, YW Chang, SI Ohba, Y Nishioka, M Kawada, H Harada, Y Kato
    Oncotarget, 2018;9(32):22480-22497.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
    Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
    PLoS ONE, 2017;12(6):e0179257.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Loss of podocalyxin causes a novel syndromic type of congenital nephrotic syndrome
    Authors: HG Kang, M Lee, KB Lee, M Hughes, BS Kwon, S Lee, KM McNagny, YH Ahn, JM Ko, IS Ha, M Choi, HI Cheong
    Exp. Mol. Med., 2017;49(12):e414.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells
    Nat Protoc, 2017;12(1):195-207.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. ?-Catenin Is Required for Endothelial Cyp1b1 Regulation Influencing Metabolic Barrier Function
    J Neurosci, 2016;36(34):8921-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  10. Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies.
    Authors: Ylostalo J, Bazhanov N, Prockop DJ
    Exp. Hematol., 2008;36(10):1390-402.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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