|Detection of Human Podocalyxin by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Podocalyxin in Human Kidney. Podocalyxin was detected in immersion fixed paraffin-embedded sections of human kidney using 5 µg/mL Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
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