Peroxisome proliferator-activated receptor gamma (PPAR gamma) is a lipid-activated nuclear hormone receptor with roles in adipocyte differentiation, lipid and glucose metabolism, and modulation of immune and inflammatory reactions. PPAR gamma is the receptor for insulin-sensitizing thiazolidinediones, commonly used for the treatment of type 2 diabetes.
Human PPAR gamma 2/NR1C3 Antibody (Clone K8450B)
R&D Systems | Catalog # PP-K8450B-00
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot, Direct ELISA, Gel Supershift Assay
Cited:
Western Blot, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # K8450B
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Product Specifications
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human PPAR gamma 2/NR1C3 Antibody (Clone K8450B)
Application
Recommended Usage
Direct ELISA
Optimal dilutions of this antibody should be experimentally determined.
Gel Supershift Assay
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: PPAR gamma/NR1C3
Long Name
Peroxisome Proliferator-activated Receptor gamma
Alternate Names
NR1C3, PPARG
Entrez Gene IDs
5468 (Human)
Gene Symbol
PPARG
Additional PPAR gamma/NR1C3 Products
Product Documents for Human PPAR gamma 2/NR1C3 Antibody (Clone K8450B)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human PPAR gamma 2/NR1C3 Antibody (Clone K8450B)
For research use only
Citations for Human PPAR gamma 2/NR1C3 Antibody (Clone K8450B)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
TGF-beta Signaling Pathways
