Human/Primate BCMA/TNFRSF17 Antibody AF193: R&D Systems

Human/Primate BCMA/TNFRSF17 Antibody

(4 citations)   
  • Species Reactivity
    Human, Primate
  • Specificity
    Detects human BCMA in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhTACI, and recombinant mouse BCMA is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BCMA/TNFRSF17
    Met1-Ala54
    Accession # Q6PE46
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 0.3-1.2 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human APRIL/TNFSF13 (Catalog # 884-AP) to immobilized Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC) coated at 1 µg/mL (100 µL/well). At 15 μg/mL, this antibody will block >90% of the binding.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Human/Primate BCMA/TNFRSF17 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human/Primate BCMA/TNFRSF17 Antibody (Catalog # AF193)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human/Primate BCMA/TNFRSF17 Biotinylated Antibody (Catalog # BAF193)
  • ELISA Standard
     
    Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF (Catalog # 193-BC)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of BCMA/TNFRSF17 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Goat Anti-Human/Primate BCMA/
TNFRSF17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF193, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BCMA/TNFRSF17

BCMA, B cell maturation, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

  • References:
    1. Madry, C. et al. (1998) Int. Immunol. 10:1693.
    2. Gras, M. et al. (1995) Int. Immunol. 7:1093.
    3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
    4. Marsters, S. et al. (2000) Curr. Biol. 10:785.
    5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.
  • Long Name:
    B Cell Maturation Factor
  • Entrez Gene IDs:
    608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)
  • Alternate Names:
    B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF17; tumor necrosis factor receptor superfamily, member 17
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2016;30(1):163-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. B-cell Maturation Antigen is a Promising Target for Adoptive T-cell Therapy of Multiple Myeloma.
    Authors: Carpenter, Robert O, Evbuomwan, Moses O, Pittaluga, Stefania, Rose, Jeremy J, Raffeld, Mark, Yang, Shicheng, Gress, Ronald E, Hakim, Frances, Kochenderfer, James N
    Clin Cancer Res, 2013;19(0):2048-60.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Aberrant expression of BAFF by B lymphocytes infiltrating the salivary glands of patients with primary Sjögren's syndrome.
    Authors: Daridon C, Devauchelle V, Hutin P, Le Berre R, Martins-Carvalho C, Bendaoud B, Dueymes M, Saraux A, Youinou P, Pers JO
    Arthritis Rheum., 2007;56(4):1134-44.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Role of B-cell-activating factor in adhesion and growth of human multiple myeloma cells in the bone marrow microenvironment.
    Authors: Tai YT, Li XF, Breitkreutz I, Song W, Neri P, Catley L, Podar K, Hideshima T, Chauhan D, Raje N, Schlossman R, Richardson P, Munshi NC, Anderson KC
    Cancer Res., 2006;66(13):6675-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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