Human/Primate CCL5/RANTES Antibody
1 Image
Summary
Data Examples
Preparation and Storage
Reconstitution Calculator
Background
Product Datasheets
Related Research Areas
Human/Primate CCL5/RANTES Antibody Summary
Species Reactivity
Human, Primate
Specificity
Detects human and primate CCL5/RANTES in direct ELISAs and Western blots. When used in combination with the biotinylated anti-human/primate CCL5/RANTES detection antibody (Catalog # BAF278) in sandwich ELISAs, no significant cross-reactivity or interference is observed with recombinant human (rh) ANG, rhCNTF, rh beta -ECGF, rhEGF, mouse (m) EGF, rhEpo, bovine (b) FGF acidic, rhFGF acidic, bFGF basic, rhFGF basic, rhFGF-4, rhG-CSF, rhGM-CSF, recombinant mouse (rm) GM-CSF, rhGRO alpha, rhIFN-gamma, rhIGF-I, rhIGF-II, rhIL-1 alpha, rmIL-1 alpha, rhIL-1 beta, rmIL‑1 beta, rhIL-1ra, recombinant rat (rr) IL-1ra, rhIL-2, rhIL-3, rmIL-3, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL‑10, rhIL-11, rhLIF, rhMCP‑1, rhM-CSF, rhMIP-1 alpha, rmMIP-1 alpha, rhMIP-1 beta, rmMIP-1 beta, rhOSM, human (h) PDGF, pPDGF, rhPDGF-AA, rhPDGF‑AB, rhPDGF-BB, rhSCF, rmSCF, rhSLPI, rhTGF-alpha, pTGF-beta 1, hTGF-beta 1, rhTGF-beta 1, pTGF-beta 2, rhTGF-beta 2, recombinant canine TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-alpha, rmTNF-alpha, or rmTNF-beta.
Source
Monoclonal Mouse IgG1 Clone # 21418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CCL5/RANTES
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated
Applications
Recommended Concentration
Sample
Human/Primate CCL5/RANTES Sandwich Immunoassay
Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL
Neutralization
Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.04-0.12 µg/mL in the presence of 10 ng/mL Recombinant Human CCL5/RANTES.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Neutralization
View Larger
View Larger
Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody. Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate CCL5/RANTES Monoclonal Antibody (Catalog # MAB678). The ND50 is typically 0.04-0.12 µg/mL.
Reconstitution Calculator
Reconstitution Calculator
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL5/RANTES
CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily. It binds and activates the chemokine receptors CCR1, 3 and 5.
Entrez Gene IDs
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
Product Datasheets
Citations for Human/Primate CCL5/RANTES Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
28
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Effect of teriparatide on drug treatment of tuberculous spondylitis: an experimental study
Authors: S Lee, YJ Seo, JY Choi, X Che, HJ Kim, SY Eum, MS Hong, SK Lee, DC Cho
Scientific Reports, 2022-12-15;12(1):21667.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
Cancer-associated fibroblast-derived CCL5 contributes to cisplatin resistance in A549 NSCLC cells partially through upregulation of lncRNA HOTAIR expression
Authors: X Sun, Z Chen
Oncology Letters, 2021-08-02;22(4):696.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma
Authors: YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang
Nature Communications, 2021-07-02;12(1):4091.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Immune-Stimulatory Effects of Curcumin on the Tumor Microenvironment in Head and Neck Squamous Cell Carcinoma
Authors: C Kötting, L Hofmann, R Lotfi, D Engelhardt, S Laban, PJ Schuler, TK Hoffmann, C Brunner, MN Theodoraki
Cancers, 2021-03-16;13(6):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
T cell-inducing vaccine durably prevents mucosal SHIV infection even with lower neutralizing antibody titers
Authors: PS Arunachala, TP Charles, V Joag, VS Bollimpell, MKD Scott, F Wimmers, SL Burton, CC Labranche, C Petitdeman, S Gangadhara, TM Styles, CF Quarnstrom, KA Walter, TJ Ketas, T Legere, PB Jagadeesh, SP Kasturi, A Tsai, BZ Yeung, S Gupta, M Tomai, J Vasilakos, GM Shaw, CY Kang, JP Moore, S Subramania, P Khatri, D Montefiori, PA Kozlowski, CA Derdeyn, E Hunter, D Masopust, RR Amara, B Pulendran
Nat. Med., 2020-05-11;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
DSE regulates the malignant characters of hepatocellular carcinoma cells by modulating CCL5/CCR1 axis
Authors: WC Liao, HR Yen, CK Liao, TJ Tseng, CT Lan, CH Liu
Am J Cancer Res, 2019-02-01;9(2):347-362.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Distinct susceptibility of HIV vaccine vector-induced CD4 T cells to HIV infection
Authors: S Auclair, F Liu, Q Niu, W Hou, G Churchyard, C Morgan, N Frahm, S Nitayaphan, P Pitisuthit, S Rerks-Ngar, JT Kimata, L Soong, G Franchini, M Robb, J Kim, N Michael, H Hu
PLoS Pathog., 2018-02-23;14(2):e1006888.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
C-C motif ligand 5 promotes migration of prostate cancer cells in the prostate cancer bone metastasis microenvironment
Authors: S Urata, K Izumi, K Hiratsuka, A Maolake, A Natsagdorj, K Shigehara, H Iwamoto, S Kadomoto, T Makino, R Naito, Y Kadono, WJ Lin, G Wufuer, K Narimoto, A Mizokami
Cancer Sci., 2018-02-14;109(3):724-731.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
SPON2 Promotes M1-like Macrophage Recruitment and Inhibits Hepatocellular Carcinoma Metastasis by Distinct Integrin-Rho GTPase-Hippo Pathways
Authors: YL Zhang, Q Li, XM Yang, F Fang, J Li, YH Wang, Q Yang, L Zhu, HZ Nie, XL Zhang, MX Feng, SH Jiang, GA Tian, LP Hu, HY Lee, SJ Lee, Q Xia, ZG Zhang
Cancer Res., 2018-02-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
SPARCL1 suppresses osteosarcoma metastasis and recruits macrophages by activation of canonical WNT/?-catenin signaling through stabilization of the WNT-receptor complex
Authors: SJ Zhao, YQ Jiang, NW Xu, Q Li, Q Zhang, SY Wang, J Li, YH Wang, YL Zhang, SH Jiang, YJ Wang, YJ Huang, XX Zhang, GA Tian, CC Zhang, YY Lv, M Dai, F Liu, R Zhang, D Zhou, ZG Zhang
Oncogene, 2017-10-30;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CD8+ T cells promote proliferation of benign prostatic hyperplasia epithelial cells under low androgen level via modulation of CCL5/STAT5/CCND1 signaling pathway
Authors: Y Yang, S Hu, J Liu, Y Cui, Y Fan, T Lv, L Liu, J Li, Q He, W Han, W Yu, Y Sun, J Jin
Sci Rep, 2017-02-20;7(0):42893.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
Authors: CM Henry, SJ Martin
Mol. Cell, 2017-02-16;65(4):715-729.e5.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
Authors: Osei-Kumah A, Smith R, Clifton VL
Cytokine, 2008-07-17;43(2):187-93.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Relation of circulating concentrations of chemokine receptor CCR5 ligands to C-peptide, proinsulin and HbA1c and disease progression in type 1 diabetes.
Authors: Pfleger C, Kaas A, Hansen L, Alizadeh B, Hougaard P, Holl R, Kolb H, Roep BO, Mortensen HB, Schloot NC
Clin. Immunol., 2008-04-22;128(1):57-65.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development -
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
Int. J. Cancer, 2008-02-15;122(4):769-76.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clin. Chem., 2007-11-01;53(11):2010-2.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Ultrasensitive flow-based immunoassays using single-molecule counting.
Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clin. Chem., 2007-09-21;53(11):1990-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood, 2007-02-08;109(10):4272-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES.
Authors: Tikhonov I, Deetz CO, Paca R, Berg S, Lukyanenko V, Lim JK, Pauza CD
Int. Immunol., 2006-06-01;18(8):1243-51.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Mass Spectometry -
Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression.
Authors: Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY
Immunol. Lett., 2006-03-03;105(2):159-66.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
Authors: Edwards MR, Johnson MW, Johnston SL
Am. J. Respir. Cell Mol. Biol., 2006-01-19;34(5):616-24.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression.
Authors: Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B
J. Virol., 2006-01-01;80(1):236-45.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
J. Immunol., 2002-11-01;169(9):5244-51.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
Authors: Hirst SJ, Hallsworth MP, Peng Q
Am. J. Respir. Crit. Care Med., 2002-04-15;165(8):1161-71.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
Immunity, 2001-06-01;14(6):705-14.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Chemoattractant factors in breast milk from allergic and nonallergic mothers.
Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
Pediatr. Res., 2000-05-01;47(5):592-7.
Species: Human
Sample Types: Serum
Applications: ELISA Development
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human/Primate CCL5/RANTES Antibody
Average Rating: 4.5 (Based on 4 Reviews)
Have you used Human/Primate CCL5/RANTES Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Human/Primate CCL5/RANTES Antibody
By Katie Myers
on 11/28/2022
Application: ELISA
Sample Tested: Serum
Species:
Primate
Used as a capture in our NHP ELISA
Human/Primate CCL5/RANTES Antibody
By Anonymous
on 07/24/2018
Application: ELISA
Sample Tested: EDTA Plasma
Species:
Human
Human/Primate CCL5/RANTES Antibody
By Balaji Mahender
on 12/06/2017
Application: ELISA
Sample Tested: EDTA Plasma
Species:
Human
Human/Primate CCL5/RANTES Antibody
By Anonymous
on 11/22/2017
Application: ELISA
Sample Tested: Serum and Plasma
Species:
Human
The RANTES ELISA we made used MAG678 as the capture and BAF278 as the detection. Dilutional linearity of samples wasn't great but otherwise the assay worked very well.
