Human Pro‑Cathepsin B Antibody
Human Pro‑Cathepsin B Antibody Summary
Accession # P07858
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Human Cathepsin B Sandwich Immunoassay
Human Pro-Cathepsin B Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin B
Cathepsin B is the first described member of the family of lysosomal cysteine proteases (1). Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin and secretory leucoprotease inhibitor (SLPI) (2 - 4). Therefore, Cathepsin B may play a role in activation and inactivation of caspases, activation of renin and inactivation of SLPI, the key steps in apoptosis, angiotensin production, and progression of emphysema, respectively. Because of its increased levels and redistribution of the enzyme in human and animal tumors, Cathepsin B may also have role in invasion and metastasis (5).
In addition to lysosome, Cathepsin B can be secreted or associated with plasma membrane, cytoplasm, and nucleus. It is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme (1).
- Mort, J.S. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds): Academic Press, San Diego, p. 1079.
- Vancompernolle, K. et al. (1998) FEBS Lett. 438:150.
- Jutras, I. and T.L. Reudelhuber (1999) FEBS Lett. 443:48.
- Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
- Bergquin, I.M. and B.F. Sloane (1996) Adv. Exp. Med. Biol. 389:281.
Citation for Human Pro‑Cathepsin B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
P2X(7) receptor-mediated release of cathepsins from macrophages is a cytokine-independent mechanism potentially involved in joint diseases.
Authors: Lopez-Castejon G, Theaker J, Pelegrin P
J. Immunol., 2010;185(4):2611-9.
Sample Types: Cell Culture Supernates
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