Human Pro MMP-7 Antibody (6A4)

R&D Systems | Catalog # MAB907

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6A4
View all MMP-7 Primary Antibodies »

Product Specifications

Immunogen

Human MMP-7 synthetic peptide
AEYSLFPNSPKWTSKV, Ala93-Val108
Accession # P09237

Specificity

Detects recombinant human (rh) Pro-MMP-7 (aa 18-267) in Western blots. In Western blots, no cross-reactivity with rhMMP-1, rhMMP-2, rhMMP-9 or mature rhMMP-7 (aa 95-267) is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Pro MMP-7 Antibody (6A4)

MMP‑7 antibody in Human Pancreatic Cancer Tissue by Immunohistochemistry (IHC-P).

MMP‑7 in Human Pancreatic Cancer Tissue.

MMP-7 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Human Pro-MMP-7 Monoclonal Antibody (Catalog # MAB907) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Pro MMP-7 Antibody (6A4)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (neuroblastoma) and human pancreatic cancer tissue

Western Blot

1 µg/mL
Sample: Recombinant Human MMP‑7 Western Blot Standard (Catalog # WBC016)

Reviewed Applications

Read 1 review rated 5 using MAB907 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-7

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha -defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

Long Name

Matrix Metalloproteinase 7

Alternate Names

Matrilysin, MMP7

Entrez Gene IDs

4316 (Human); 17393 (Mouse)

Gene Symbol

MMP7

UniProt

P09237

Additional MMP-7 Products

Product Documents for Human Pro MMP-7 Antibody (6A4)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Pro MMP-7 Antibody (6A4)

For research use only

Citations for Human Pro MMP-7 Antibody (6A4)

Customer Reviews for Human Pro MMP-7 Antibody (6A4) (1)

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  • Human Pro‑MMP‑7 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 05/28/2022
    Human Pro MMP-7 Antibody (6A4) MAB907

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