Human Proteinase 3/Myeloblastin/PRTN3 Antibody Summary
Accession # P24158
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Proteinase 3/Myeloblastin/PRTN3 in Human Granulocytes. Proteinase 3/ Myeloblastin/PRTN3 was detected in immersion fixed human granulocytes using Mouse Anti-Human Proteinase 3/ Myeloblastin/PRTN3 Monoclonal Antibody (Catalog # MAB61341) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Proteinase 3/Myeloblastin/PRTN3
Leukocyte proteinase 3 (PRTN3 or PR3), also called Myeloblastin (MBN) or NP-4, is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human MBN proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221 aa mature enzyme (aa 28-248), and an eight aa C-terminal propeptide (aa 249-256). Within the cell, a 35 kDa immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa 26-249, human MBN shares 68% aa sequence identity with mouse MBN.
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