Human Protocadherin-15 Antibody

Catalog # Availability Size / Price Qty
MAB6729
MAB6729-SP

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Protocadherin‑15 in YT Human Cell Line.
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Human Protocadherin-15 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Protocadherin-15 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 740523
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Protocadherin-15
Tyr28-Ala1376
Accession # Q96QU1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Complete Your Experiment

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Protocadherin‑15 in YT Human Cell Line. View Larger

Protocadherin‑15 in YT Human Cell Line. Protocadherin-15 was detected in immersion fixed YT human leukemia natural killer-like cell line using Mouse Anti-Human Protocadherin-15 Monoclonal Antibody (Catalog # MAB6729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-15

Protocadherin-15 (PCDH-15) is a 250-270 kDa "other" member of the nonclustered group of the protocadherin family of molecules. In the adult, it is expressed on the base of hair cell stereocilia in the Organ of Corti, on cerebellar granule cells, and on rod and cone photoreceptors. In the ear, it is expressed as a homodimer, and binds (in trans) to cadherin 23 homodimers on adjacent stereocilia. Mature human PCDH-15 is a type I transmembrane protein that is 1929 amino acids (aa) in length. It contains a 1350 aa extracellular domain (ECD) (aa 27-1376) plus a 558 aa cytoplasmic region. There are 11 cadherin domains in the ECD (aa 40-1259). Multiple splice forms are reported. One is a soluble 110 kDa form that shows a five aa substitution for aa 957-1955. Another contains a seven aa substitution for aa 1119-1125. Others show substitutions or deletions in either the extracellular or cytoplasmic domains. Over aa 27-1376 (the ECD), human PCDH-15 shares 95% aa sequence identity with mouse PCDH-15.

Entrez Gene IDs
65217 (Human); 11994 (Mouse); 690865 (Rat)
Alternate Names
autosomal recessive 23; cadherin-related family member 15; CDHR15; DFNB23; DKFZp667A1711; PCDH15; Protocadherin15; Protocadherin-15; protocadherin-related 15; USH1F

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Protocadherin-15 Antibody

Average Rating: 4.3 (Based on 3 Reviews)

5 Star
33.33%
4 Star
66.67%
3 Star
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2 Star
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1 Star
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Human Protocadherin-15 Antibody
By Anonymous on 11/03/2022
Application: ICC/IF Sample Tested: YT Human Cell Line Species: Human

Human Protocadherin-15 Antibody
By Anonymous on 12/06/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human Protocadherin-15 Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human and Mouse