Human Prox1 Antibody

  (41 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Citations (41)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human Prox1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Prox1
    Pro2-Gln259
    Accession # Q92786
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Prox1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Prox1 at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Prox1 in HepG2 Human Hepatocellular Carcinoma Cell Line. Prox1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Prox1 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Prox1 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prox1
Prox1, the homologue of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence homology with mouse and rat Prox1.
  • Long Name:
    Prospero-related Homeobox 1
  • Entrez Gene IDs:
    5629 (Human)
  • Alternate Names:
    Homeobox prospero-like protein PROX1; prospero homeobox 1; prospero homeobox protein 1; prospero-related homeobox 1; Prox1; PROX-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program
    Authors: M Frye, A Taddei, C Dierkes, I Martinez-C, M Fielden, H Ortsäter, J Kazenwadel, DP Calado, P Ostergaard, M Salminen, L He, NL Harvey, F Kiefer, T Mäkinen
    Nat Commun, 2018;9(1):1511.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Neurogliaform cortical interneurons derive from cells in the preoptic area
    Authors: M Niquille, G Limoni, F Markopoulo, C Cadilhac, J Prados, A Holtmaat, A Dayer
    Elife, 2018;7(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-Fr
  3. Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Authors: S Rhee, JI Chung, DA King, G D'amato, DT Paik, A Duan, A Chang, D Nagelberg, B Sharma, Y Jeong, M Diehn, JC Wu, AJ Morrison, K Red-Horse
    Nat Commun, 2018;9(1):368.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm's Canal Reporter Rat
    Authors: E Jung, D Gardner, D Choi, E Park, Y Jin Seong, S Yang, J Castorena-, A Louveau, Z Zhou, GK Lee, DP Perrault, S Lee, M Johnson, G Daghlian, M Lee, Y Jin Hong, Y Kato, J Kipnis, MJ Davis, AK Wong, YK Hong
    Sci Rep, 2017;7(1):5577.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  5. Fluorescence and Bioluminescence Imaging of Angiogenesis in Flk1-Nano-lantern Transgenic Mice
    Authors: J Matsushita, S Inagaki, T Nishie, T Sakasai, J Tanaka, C Watanabe, KI Mizutani, Y Miwa, K Matsumoto, K Takara, H Naito, H Kidoya, N Takakura, T Nagai, S Takahashi, M Ema
    Sci Rep, 2017;7(0):46597.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  6. Human umbilical cord plasma proteins revitalize hippocampal function in aged mice
    Authors: JM Castellano, KI Mosher, RJ Abbey, AA McBride, ML James, D Berdnik, JC Shen, B Zou, XS Xie, M Tingle, IV Hinkson, MS Angst, T Wyss-Coray
    Nature, 2017;544(7651):488-492.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
    Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
    PLoS ONE, 2017;12(6):e0179257.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity
    Authors: KS Yan, O Gevaert, GXY Zheng, B Anchang, CS Probert, KA Larkin, PS Davies, ZF Cheng, JS Kaddis, A Han, K Roelf, RI Calderon, E Cynn, X Hu, K Mandleywal, J Wilhelmy, SM Grimes, DC Corney, SC Boutet, JM Terry, P Belgrader, SB Ziraldo, TS Mikkelsen, F Wang, RJ von Furste, NR Smith, P Chandrakes, R May, MAS Chrissy, R Jain, CA Cartwright, JC Niland, YK Hong, J Carrington, DT Breault, J Epstein, CW Houchen, JP Lynch, MG Martin, SK Plevritis, C Curtis, HP Ji, L Li, SJ Henning, MH Wong, CJ Kuo
    Cell Stem Cell, 2017;21(1):78-90.e6.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1
    Authors: SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch
    Sci Rep, 2017;7(1):4916.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
    Authors: J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh
    J. Clin. Invest., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Dynamics and function of CXCR4 in formation of the granule cell layer during hippocampal development
    Authors: Y Mimura-Yam, H Shinohara, T Kashiwagi, T Sato, S Shioda, T Seki
    Sci Rep, 2017;7(1):5647.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  12. Transcriptomic and anatomic parcellation of 5-HT3AR expressing cortical interneuron subtypes revealed by single-cell RNA sequencing
    Authors: S Frazer, J Prados, M Niquille, C Cadilhac, F Markopoulo, L Gomez, U Tomasello, L Telley, A Holtmaat, D Jabaudon, A Dayer
    Nat Commun, 2017;8(0):14219.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  13. Arf6 in lymphatic endothelial cells regulates lymphangiogenesis by controlling directional cell migration
    Authors: YC Lin, N Ohbayashi, T Hongu, N Katagiri, Y Funakoshi, H Lee, Y Kanaho
    Sci Rep, 2017;7(1):11431.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  14. High PROX1 expression in gastric cancer predicts better survival
    Authors: A Laitinen, C Böckelman, J Hagström, A Kokkola, P Kallio, C Haglund
    PLoS ONE, 2017;12(8):e0183868.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  15. VIPAR, a quantitative approach to 3D histopathology applied to lymphatic malformations
    Authors: R Hägerling, D Drees, A Scherzinge, C Dierkes, S Martin-Alm, S Butz, K Gordon, M Schäfers, K Hinrichs, P Ostergaard, D Vestweber, T Goerge, S Mansour, X Jiang, PS Mortimer, F Kiefer
    JCI Insight, 2017;2(16):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC-Fr
  16. The role of prospero homeobox 1 (PROX1) expression in follicular thyroid carcinoma cells
    Authors: M Rudzinska, JK Ledwon, D Gawel, J Sikorska, B Czarnocka
    Oncotarget, 2017;8(69):114136-114155.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  17. BIG1 is required for the survival of deep layer neurons, neuronal polarity, and the formation of axonal tracts between the thalamus and neocortex in developing brain
    Authors: JJ Teoh, T Iwano, M Kunii, N Atik, E Avriyanti, SI Yoshimura, K Moriwaki, A Harada
    PLoS ONE, 2017;12(4):e0175888.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  18. Peg3/PW1 is a marker of a subset of vessel associated endothelial progenitors
    Authors: M Malinverno, M Corada, L Ferrarini, L Formicola, G Marazzi, D Sassoon, E Dejana
    Stem Cells, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  19. Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves
    Authors: Boksik Cha
    Genes Dev, 2016;30(12):1454-69.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  20. Foxc1 and Foxc2 deletion causes abnormal lymphangiogenesis and correlates with ERK hyperactivation
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  21. Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
    Authors: Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R
    Dev Biol, 2016;409(1):218-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  22. Lymphatic vessels regulate immune microenvironments in human and murine melanoma
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  23. CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  24. Gene-expression profiling of different arms of lymphatic vasculature identifies candidates for manipulation of cell traffic
    Proc Natl Acad Sci USA, 2016;113(38):10643-10648.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  25. Comprehensive Expression of Wnt Signaling Pathway Genes during Development and Maturation of the Mouse Cochlea.
    Authors: Geng R, Noda T, Mulvaney J, Lin V, Edge A, Dabdoub A
    PLoS ONE, 2015;11(2):e0148339.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  26. A dural lymphatic vascular system that drains brain interstitial fluid and macromolecules.
    Authors: Aspelund A, Antila S, Proulx S, Karlsen T, Karaman S, Detmar M, Wiig H, Alitalo K
    J Exp Med, 2015;212(7):991-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  27. A transgenic Prox1-Cre-tdTomato reporter mouse for lymphatic vessel research.
    Authors: Bianchi R, Teijeira A, Proulx S, Christiansen A, Seidel C, Rulicke T, Makinen T, Hagerling R, Halin C, Detmar M
    PLoS ONE, 2015;10(4):e0122976.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  28. Angiopoietin 2 regulates the transformation and integrity of lymphatic endothelial cell junctions.
    Authors: Zheng W, Nurmi H, Appak S, Sabine A, Bovay E, Korhonen E, Orsenigo F, Lohela M, D'Amico G, Holopainen T, Leow C, Dejana E, Petrova T, Augustin H, Alitalo K
    Genes Dev, 2014;28(14):1592-603.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  29. The Schlemm's canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel.
    Authors: Aspelund A, Tammela T, Antila S, Nurmi H, Leppanen V, Zarkada G, Stanczuk L, Francois M, Makinen T, Saharinen P, Immonen I, Alitalo K
    J Clin Invest, 2014;124(9):3975-86.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  30. Bone marrow-derived cells are implicated as a source of lymphatic endothelial progenitors in human breast cancer.
    Authors: Van't Hull E, Bron S, Henry L, Ifticene-Treboux A, Turrini R, Coukos G, Delaloye J, Doucey M
    Oncoimmunology, 2014;3(0):e29080.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  31. Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
    Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
    J Clin Invest, 2014;124(9):3960-74.
  32. Murine Notch1 is required for lymphatic vascular morphogenesis during development.
    Authors: Fatima A, Culver A, Culver F, Liu T, Dietz W, Thomson B, Hadjantonakis A, Quaggin S, Kume T
    Dev Dyn, 2014;243(7):957-64.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  33. A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
    Authors: Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F
    EMBO J, 2013;32(5):629-44.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  34. The germinal matrices in the developing dentate gyrus are composed of neuronal progenitors at distinct differentiation stages.
    Authors: Sugiyama T, Osumi N, Katsuyama Y
    Dev Dyn, 2013;242(12):1442-53.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: immunohistochemistry
  35. Blimp1 (Prdm1) prevents re-specification of photoreceptors into retinal bipolar cells by restricting competence.
    Authors: Brzezinski J, Uoon Park K, Reh T
    Dev Biol, 2013;384(2):194-204.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  36. Mcl-1 and Bcl-x(L) coordinately regulate megakaryocyte survival.
    Blood, 2012;119(24):5850-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  37. Aberrant mural cell recruitment to lymphatic vessels and impaired lymphatic drainage in a murine model of pulmonary fibrosis.
    Blood, 2012;119(24):5931-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  38. CKIalpha ablation highlights a critical role for p53 in invasiveness control.
    Authors: Elyada E, Pribluda A, Goldstein RE, Morgenstern Y, Brachya G, Cojocaru G, Snir-Alkalay I, Burstain I, Haffner-Krausz R, Jung S, Wiener Z, Alitalo K, Oren M, Pikarsky E, Ben-Neriah Y
    Nature, 2011;470(7334):409-13.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  39. Expression and prognostic value of transcription factor PROX1 in colorectal cancer.
    Authors: Skog M, Bono P, Lundin M, Lundin J, Louhimo J, Linder N, Petrova TV, Andersson LC, Joensuu H, Alitalo K, Haglund CH
    Br. J. Cancer, 2011;105(9):1346-51.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  40. The tyrosine kinase inhibitor cediranib blocks ligand-induced vascular endothelial growth factor receptor-3 activity and lymphangiogenesis.
    Authors: Heckman CA, Holopainen T, Wirzenius M, Keskitalo S, Jeltsch M, Yla-Herttuala S, Wedge SR, Jurgensmeier JM, Alitalo K
    Cancer Res., 2008;68(12):4754-62.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  41. Smoothened signaling in vertebrates is facilitated by a G protein-coupled receptor kinase.
    Authors: Philipp M, Fralish GB, Meloni AR, Chen W, MacInnes AW, Barak LS, Caron MG
    Mol. Biol. Cell, 2008;19(12):5478-89.
    Species: Zebrafish
    Sample Type: Whole Tissue
    Application: IHC
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human Prox1 Antibody AF2727 Array AF2727
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 ICC/IF Mouse Anonymous 08/10/2017
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Immunocytochemistry/Immunofluorescence Human Prox1 Antibody AF2727
Immunocytochemistry/Immunofluorescence: Human Prox1 Antibody [AF2727].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested Embryonic kidney
Species Mouse

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