Human Prox1 Antibody

(34 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Prox1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Prox1
    Pro2-Gln259
    Accession # Q92786
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Prox1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Prox1 at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Prox1 in HepG2 Human Hepatocellular Carcinoma Cell Line. Prox1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Prox1 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Prox1 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prox1
Prox1, the homologue of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence homology with mouse and rat Prox1.
  • Long Name:
    Prospero-related Homeobox 1
  • Entrez Gene IDs:
    5629 (Human)
  • Alternate Names:
    Homeobox prospero-like protein PROX1; prospero homeobox 1; prospero homeobox protein 1; prospero-related homeobox 1; Prox1; PROX-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
    Authors: J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh
    J. Clin. Invest., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Human umbilical cord plasma proteins revitalize hippocampal function in aged mice
    Authors: JM Castellano, KI Mosher, RJ Abbey, AA McBride, ML James, D Berdnik, JC Shen, B Zou, XS Xie, M Tingle, IV Hinkson, MS Angst, T Wyss-Coray
    Nature, 2017;544(7651):488-492.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Peg3/PW1 is a marker of a subset of vessel associated endothelial progenitors
    Authors: M Malinverno, M Corada, L Ferrarini, L Formicola, G Marazzi, D Sassoon, E Dejana
    Stem Cells, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  4. BIG1 is required for the survival of deep layer neurons, neuronal polarity, and the formation of axonal tracts between the thalamus and neocortex in developing brain
    Authors: JJ Teoh, T Iwano, M Kunii, N Atik, E Avriyanti, SI Yoshimura, K Moriwaki, A Harada
    PLoS ONE, 2017;12(4):e0175888.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  5. Transcriptomic and anatomic parcellation of 5-HT3AR expressing cortical interneuron subtypes revealed by single-cell RNA sequencing
    Authors: S Frazer, J Prados, M Niquille, C Cadilhac, F Markopoulo, L Gomez, U Tomasello, L Telley, A Holtmaat, D Jabaudon, A Dayer
    Nat Commun, 2017;8(0):14219.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. Fluorescence and Bioluminescence Imaging of Angiogenesis in Flk1-Nano-lantern Transgenic Mice
    Authors: J Matsushita, S Inagaki, T Nishie, T Sakasai, J Tanaka, C Watanabe, KI Mizutani, Y Miwa, K Matsumoto, K Takara, H Naito, H Kidoya, N Takakura, T Nagai, S Takahashi, M Ema
    Sci Rep, 2017;7(0):46597.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  7. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
    Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
    PLoS ONE, 2017;12(6):e0179257.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity
    Authors: KS Yan, O Gevaert, GXY Zheng, B Anchang, CS Probert, KA Larkin, PS Davies, ZF Cheng, JS Kaddis, A Han, K Roelf, RI Calderon, E Cynn, X Hu, K Mandleywal, J Wilhelmy, SM Grimes, DC Corney, SC Boutet, JM Terry, P Belgrader, SB Ziraldo, TS Mikkelsen, F Wang, RJ von Furste, NR Smith, P Chandrakes, R May, MAS Chrissy, R Jain, CA Cartwright, JC Niland, YK Hong, J Carrington, DT Breault, J Epstein, CW Houchen, JP Lynch, MG Martin, SK Plevritis, C Curtis, HP Ji, L Li, SJ Henning, MH Wong, CJ Kuo
    Cell Stem Cell, 2017;21(1):78-90.e6.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1
    Authors: SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch
    Sci Rep, 2017;7(1):4916.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm's Canal Reporter Rat
    Authors: E Jung, D Gardner, D Choi, E Park, Y Jin Seong, S Yang, J Castorena-, A Louveau, Z Zhou, GK Lee, DP Perrault, S Lee, M Johnson, G Daghlian, M Lee, Y Jin Hong, Y Kato, J Kipnis, MJ Davis, AK Wong, YK Hong
    Sci Rep, 2017;7(1):5577.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  11. Dynamics and function of CXCR4 in formation of the granule cell layer during hippocampal development
    Authors: Y Mimura-Yam, H Shinohara, T Kashiwagi, T Sato, S Shioda, T Seki
    Sci Rep, 2017;7(1):5647.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  12. Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
    Authors: Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R
    Dev Biol, 2016;409(1):218-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  13. Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves
    Authors: Boksik Cha
    Genes Dev, 2016;30(12):1454-69.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  14. Lymphatic vessels regulate immune microenvironments in human and murine melanoma
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  15. CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. Foxc1 and Foxc2 deletion causes abnormal lymphangiogenesis and correlates with ERK hyperactivation
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  17. Gene-expression profiling of different arms of lymphatic vasculature identifies candidates for manipulation of cell traffic
    Proc Natl Acad Sci USA, 2016;113(38):10643-10648.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  18. A transgenic Prox1-Cre-tdTomato reporter mouse for lymphatic vessel research.
    Authors: Bianchi R, Teijeira A, Proulx S, Christiansen A, Seidel C, Rulicke T, Makinen T, Hagerling R, Halin C, Detmar M
    PLoS ONE, 2015;10(4):e0122976.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  19. A dural lymphatic vascular system that drains brain interstitial fluid and macromolecules.
    Authors: Aspelund A, Antila S, Proulx S, Karlsen T, Karaman S, Detmar M, Wiig H, Alitalo K
    J Exp Med, 2015;212(7):991-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  20. Comprehensive Expression of Wnt Signaling Pathway Genes during Development and Maturation of the Mouse Cochlea.
    Authors: Geng R, Noda T, Mulvaney J, Lin V, Edge A, Dabdoub A
    PLoS ONE, 2015;11(2):e0148339.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  21. Bone marrow-derived cells are implicated as a source of lymphatic endothelial progenitors in human breast cancer.
    Authors: Van't Hull E, Bron S, Henry L, Ifticene-Treboux A, Turrini R, Coukos G, Delaloye J, Doucey M
    Oncoimmunology, 2014;3(0):e29080.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  22. Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
    Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
    J Clin Invest, 2014;124(9):3960-74.
  23. The Schlemm's canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel.
    Authors: Aspelund A, Tammela T, Antila S, Nurmi H, Leppanen V, Zarkada G, Stanczuk L, Francois M, Makinen T, Saharinen P, Immonen I, Alitalo K
    J Clin Invest, 2014;124(9):3975-86.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  24. Angiopoietin 2 regulates the transformation and integrity of lymphatic endothelial cell junctions.
    Authors: Zheng W, Nurmi H, Appak S, Sabine A, Bovay E, Korhonen E, Orsenigo F, Lohela M, D'Amico G, Holopainen T, Leow C, Dejana E, Petrova T, Augustin H, Alitalo K
    Genes Dev, 2014;28(14):1592-603.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  25. Murine Notch1 is required for lymphatic vascular morphogenesis during development.
    Authors: Fatima A, Culver A, Culver F, Liu T, Dietz W, Thomson B, Hadjantonakis A, Quaggin S, Kume T
    Dev Dyn, 2014;243(7):957-64.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  26. A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
    Authors: Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F
    EMBO J, 2013;32(5):629-44.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  27. The germinal matrices in the developing dentate gyrus are composed of neuronal progenitors at distinct differentiation stages.
    Authors: Sugiyama T, Osumi N, Katsuyama Y
    Dev Dyn, 2013;242(12):1442-53.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: immunohistochemistry
  28. Blimp1 (Prdm1) prevents re-specification of photoreceptors into retinal bipolar cells by restricting competence.
    Authors: Brzezinski J, Uoon Park K, Reh T
    Dev Biol, 2013;384(2):194-204.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  29. Mcl-1 and Bcl-x(L) coordinately regulate megakaryocyte survival.
    Blood, 2012;119(24):5850-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  30. Aberrant mural cell recruitment to lymphatic vessels and impaired lymphatic drainage in a murine model of pulmonary fibrosis.
    Blood, 2012;119(24):5931-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  31. CKIalpha ablation highlights a critical role for p53 in invasiveness control.
    Authors: Elyada E, Pribluda A, Goldstein RE, Morgenstern Y, Brachya G, Cojocaru G, Snir-Alkalay I, Burstain I, Haffner-Krausz R, Jung S, Wiener Z, Alitalo K, Oren M, Pikarsky E, Ben-Neriah Y
    Nature, 2011;470(7334):409-13.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  32. Expression and prognostic value of transcription factor PROX1 in colorectal cancer.
    Authors: Skog M, Bono P, Lundin M, Lundin J, Louhimo J, Linder N, Petrova TV, Andersson LC, Joensuu H, Alitalo K, Haglund CH
    Br. J. Cancer, 2011;105(9):1346-51.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  33. The tyrosine kinase inhibitor cediranib blocks ligand-induced vascular endothelial growth factor receptor-3 activity and lymphangiogenesis.
    Authors: Heckman CA, Holopainen T, Wirzenius M, Keskitalo S, Jeltsch M, Yla-Herttuala S, Wedge SR, Jurgensmeier JM, Alitalo K
    Cancer Res., 2008;68(12):4754-62.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  34. Smoothened signaling in vertebrates is facilitated by a G protein-coupled receptor kinase.
    Authors: Philipp M, Fralish GB, Meloni AR, Chen W, MacInnes AW, Barak LS, Caron MG
    Mol. Biol. Cell, 2008;19(12):5478-89.
    Species: Zebrafish
    Sample Type: Whole Tissue
    Application: IHC
Expand to show all 34 Citations
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 1 review)

Have you used Human Prox1 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human Prox1 Antibody.

We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

Filter by Ratings
Excellent
(1)
All Ratings
Filter by Applications
ICC/IF
(1)
All Applications
Filter by Species
Mouse
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human Prox1 Antibody AF2727
Enlarge
Excellent
 ICC/IF Mouse Anonymous 08/10/2017
Show
Image Details
Immunocytochemistry/Immunofluorescence Human Prox1 Antibody AF2727
Immunocytochemistry/Immunofluorescence: Human Prox1 Antibody [AF2727]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedEmbryonic kidney
SpeciesMouse

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information