Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 467657
View all Rab25 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RAB25
Met1-Ala195
Accession # P57735

Specificity

Detects human RAB25 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human RAB5A, 10, 11A, 27A, or 27B is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human RAB25 Antibody

Detection of Human RAB25 antibody by Western Blot.

Detection of Human RAB25 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, LNCaP human prostate cancer cell line, and HT-29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human RAB25 Monoclonal Antibody (Catalog # MAB6605) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RAB25 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human RAB25 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line, LNCaP human prostate cancer cell line, and HT‑29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rab25

Ras-related protein RAB25, also known as CATX-8, is a 23 kDa (unglycosylated) member of the small GTPase superfamily and RAB family of proteins. Human RAB25 is 213 amino acids (aa) in length. The mature chain runs from aa 1-210 with residues 211-213 constituting a propeptide. Residues 10-174 are noted to be RAB11-like. RAB11a, RAB11b, and RAB25 are closely related RAB proteins that are differentially expressed. Human RAB25 shares 96% aa sequence identity with mouse RAB25. Functionally, RAB25 is involved in the regulation of cell survival. Researchers have shown that RAB25 promotes invasive migration of cells and increases the rates and aggressiveness of breast and ovarian cancers. It is expressed in breast and ovarian tissues.

Long Name

RAs Genes from Brain Protein 25

Alternate Names

CATX8, RAB11C

Entrez Gene IDs

57111 (Human); 53868 (Mouse); 310632 (Rat)

Gene Symbol

RAB25

UniProt

P57735 (Human)

Additional Rab25 Products

Product Documents for Human RAB25 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human RAB25 Antibody

For research use only

Related Research Areas

Autophagy
Cancer Biomarkers

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