Human RANK/TNFRSF11A Antibody MAB6831-100: R&D Systems

Human RANK/TNFRSF11A Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects recombinant human RANK in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) 4‑1BB, rhBAFF R, recombinant mouse (rm) BAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR4, rhDR5, rhEDAR, rmEDAR, rmFas, rhGITR, rhHVEM, rhLymphotoxin beta R, rhNGF R, rhOPG, rmRANK, rhRELT, rhTNF RI, rmTNF RI, rhTNF RII, rhTRAIL R2, rhTRAIL R3, rhTRAIL R4, or rhTROY is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 80707
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human RANK
    Leu28-Gly213
    Accession # Q9Y6Q6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human RANK/TNFRSF11A Fc Chimera (Catalog # 683-RK)
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
RANK/TNFRSF11A in Human Liver. RANK/TNFRSF11A was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human RANK/TNFRSF11A Monoclonal Antibody (Catalog # MAB6831) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR]) and TNFRSF11A is a member of the tumor necrosis factor receptor family. The full length human RANK cDNA encodes a type I transmembrane protein of 616 amino acids with a predicted 184 amino acid extracellular domain and a 383 amino acid cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains. RANK is widely expressed with highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

  • References:
    1. Anderson, D.M. et al. (1997) Nature 390:175.
    2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  • Long Name:
    Receptor Activator of NF-kappa-B
  • Entrez Gene IDs:
    8792 (Human); 21934 (Mouse)
  • Alternate Names:
    CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. HER2 and EGFR overexpression support metastatic progression of prostate cancer to bone
    Cancer Res., 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  2. High expression levels of receptor activator of nuclear factor-kappa B ligand associated with human chronic periodontitis are mainly secreted by CD4+ T lymphocytes.
    Authors: Vernal R, Dutzan N, Hernandez M, Chandia S, Puente J, Leon R, Garcia L, Del Valle I, Silva A, Gamonal J
    J. Periodontol., 2006;77(10):1772-80.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
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