Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 659209
View all RARRES3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RARRES3
Asp12-Lys134
Accession # Q9UL19

Specificity

Recognizes human RARRES3 in Western blots and direct ELISAs. In Western blots and direct ELISAs, no cross-reactivity with recombinant human (rh) RARRES1 or rhLatexin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human RARRES3 Antibody

RARRES3 antibody in Raji Human Cell Line by Immunocytochemistry (ICC).

RARRES3 in Raji Human Cell Line.

RARRES3 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human RARRES3 Monoclonal Antibody (Catalog # MAB6139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human RARRES3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RARRES3

RARRES3 (retinoic acid receptor responder 3), also called Tig3 (tazarotene-induced gene 3) or RIG1 (retinoid-inducible gene 1), is an 18 kDa, 164 amino acid (aa) cytoplasmic protein within the LRAT (lecithin retinol acyltransferase) family of growth inhibitory proteins, which are class II tumor-suppressors. RARRES3 is expressed in a variety of cells including epidermal keratinocytes, and may be downregulated in tumor cells and cell lines. It shows phospholipase activity and interaction with type I transglutaminase. A C-terminal hydrophobic region allows perinuclear localization. RARRES3 is not structurally related to other RARRES genes. A canine ortholog shares 72% aa identity within the region used as an immunogen; orthologs are unlikely in rodents.

Long Name

Retinoic Acid Receptor Responder [Tazarotene-induced] 3

Alternate Names

HRASLS4, TIG3

Entrez Gene IDs

5920 (Human)

Gene Symbol

PLAAT4

UniProt

Q9UL19 (Human)

Additional RARRES3 Products

Product Documents for Human RARRES3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human RARRES3 Antibody

For research use only

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