Human RARRES3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Recognizes human RARRES3 in Western blots and direct ELISAs. In Western blots and direct ELISAs, no cross-reactivity with recombinant human (rh) RARRES1 or rhLatexin is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 659209
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human RARRES3
    Asp12-Lys134
    Accession # Q9UL19
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
RARRES3 in Raji Human Cell Line. RARRES3 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human RARRES3 Monoclonal Antibody (Catalog # MAB6139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RARRES3
RARRES3 (retinoic acid receptor responder 3), also called Tig3 (tazarotene-induced gene 3) or RIG1 (retinoid-inducible gene 1), is an 18 kDa, 164 amino acid (aa) cytoplasmic protein within the LRAT (lecithin retinol acyltransferase) family of growth inhibitory proteins, which are class II tumor-suppressors. RARRES3 is expressed in a variety of cells including epidermal keratinocytes, and may be downregulated in tumor cells and cell lines. It shows phospholipase activity and interaction with type I transglutaminase. A C-terminal hydrophobic region allows perinuclear localization. RARRES3 is not structurally related to other RARRES genes. A canine ortholog shares 72% aa identity within the region used as an immunogen; orthologs are unlikely in rodents.
  • Long Name:
    Retinoic Acid Receptor Responder [Tazarotene-induced] 3
  • Entrez Gene IDs:
    5920 (Human)
  • Alternate Names:
    HRASLS4; PLA1/2-3; RARRES3; RAR-responsive protein TIG3; retinoic acid receptor responder (tazarotene induced) 3; retinoic acid receptor responder protein 3; retinoic acid-inducible gene 1; Retinoid-inducible gene 1 protein; RIG1; Tazarotene-induced gene 3 protein; TIG3; TIG3MGC8906
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