Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 512124
View all Reg3A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Reg3A
Glu27-Asp175
Accession # Q06141

Specificity

Detects human Reg3A in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Reg3A Antibody

Detection of Human Reg3A antibody by Western Blot.

Detection of Human Reg3A by Western Blot.

Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Reg3A Monoclonal Antibody (Catalog # MAB5965) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Reg3A at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reg3A antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Reg3A in HepG2 Human Cell Line.

Reg3A was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Reg3A Mono-clonal Antibody (Catalog # MAB5965) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Reg3A Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Huh-7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line

Western Blot

2 µg/mL
Sample: Human small intestine tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Reg3A

Reg3A, also named Pancreatitis-Associated Protein (PAP) and peptide 23, belongs to the Regenerating gene family (Reg), within the superfamily of C-type lectins. It has been classified as a member of the type III subclass of the Reg family. Reg3A has anti-apoptotic effects on pancreatic acinar cells and is a novel motor and sensory neuron survival factor.

Long Name

Regenerating Islet-derived IIIa

Alternate Names

Lithostathine 3, PAP2, REG3A

Entrez Gene IDs

5068 (Human); 19694 (Mouse); 171162 (Rat)

Gene Symbol

REG3A

UniProt

Q06141 (Human)

Additional Reg3A Products

Product Documents for Human Reg3A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Reg3A Antibody

For research use only

Related Research Areas

C-type Lectin Family
Diabetes

Citations for Human Reg3A Antibody

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Protocols

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