Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 200214
View all Reg4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Reg4
Asp23-Pro158
Accession # Q9BYZ8

Specificity

Detects human Reg4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse (rm) Reg1, rmReg2, recombinant rat (rr) Reg3B, rmReg3A, rrReg3A, or rhReg1B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Reg4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Reg4 (Catalog # 1379-RG)
under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 5 using MAB1379 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Reg4

Reg4 (regenerating islet-derived protein 4; also named Reg-like protein, RELP, and gastrointestinal secretory protein) is a 17 kDa, secreted glycoprotein belonging to the calcium (C-type) dependent lectin superfamily and the regenerating gene family (1‑3). There are three potential isoforms for human Reg4. Isoform 1 is synthesized as a 158 amino acid (aa) precursor with a 22 aa signal sequence and a 136 aa mature chain. Amino acids 30‑156 constitute a C-type lectin-like domain, and aa 50 is a site for potential N-linked glycosylation. In addition, amino acids 127 and 142‑143 form a ligand-binding surface. In isoform 2, there is a 79 aa substitution for the C-terminal 101 amino acids, while in isoform 3, there is an 11 aa substitution for the C‑terminal 57 amino acids. Human Reg4 shares 68% and 67% aa sequence identity with rat and mouse Reg4, respectively. Like other members of the regenerating gene family, Reg4 is expressed in the gastrointestinal (GI) tract and ectopically at other sites in the setting of tissue injury (1, 2). In particular, Reg4 is expressed in normal colon mucosa, and up‑regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, inflammatory bowel disease (Crohn’s disease and ulcerative colitis), and, outside the GI tract, in prostate adenocarcinoma (2‑8). The physiological function of Reg4 is presently unknown, but it may be involved in inflammatory and metaplastic responses of the GI epithelium (3).

References

  1. Hartupee, J.C. et al. (2001) Biochim. Biophys. Acta 1518:287.
  2. Zhang, Y. et al. (2003) World J. Gastroenterol. 9:2635.
  3. Kamarainen, M. et al. (2003) Am. J. Pathol. 163:11.
  4. Zhang, Y. et al. (2003) Cancer Lett. 200:69.
  5. Violette, S. et al. (2003) Int. J. Cancer 103:185.
  6. Oue, N. et al. (2005) J. Pathol. 207:185.
  7. Zhennan, G. et al. (2005) Clin. Cancer Res. 2:2237
  8. Bishnupuri, K.S. et al. (2006) Gastroenterology 130:137.

Long Name

Regenerating Islet-derived 4

Alternate Names

GISP, RELP

Entrez Gene IDs

83998 (Human); 67709 (Mouse); 445583 (Rat)

Gene Symbol

REG4

UniProt

Q9BYZ8

Additional Reg4 Products

Product Documents for Human Reg4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Reg4 Antibody

For research use only

Citations for Human Reg4 Antibody

Customer Reviews for Human Reg4 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
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1 Stars
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Showing  1 - 2 of 2 reviews Showing All
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  • Human Reg4 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Reg4 Antibody MAB1379
  • Human Reg4 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 10/04/2019
    We used this antibody for a sandwich ELISA format in combination with pAb (AF1379) and protein (1379-RG). This combination worked very well and detected human Reg 4 in serum and plasma samples.
    Human Reg4 Antibody MAB1379

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