Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Reg4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Reg4
Asp23-Pro158
Accession # Q9BYZ8

Specificity

Detects human Reg4 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse (rm) Reg4, rmReg3A, and recombinant rat (rr) Reg3A is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Reg4 Antibody

Reg4 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P).

Reg4 in Human Colon Cancer Tissue.

Reg4 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Reg4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1379) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Reg4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human normal colon and colon cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Reg4 (Catalog # 1379-RG)

Reviewed Applications

Read 3 reviews rated 4.7 using AF1379 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Reg4

Reg4 (regenerating islet-derived protein 4; also named Reg-like protein, RELP, and gastrointestinal secretory protein) is a 17 kDa, secreted glycoprotein belonging to the calcium (C-type) dependent lectin superfamily and the regenerating gene family (1-3). There are three potential isoforms for human Reg4. Isoform 1 is synthesized as a 158 amino acid (aa) precursor with a 22 aa signal sequence and a 136 aa mature chain. Amino acids 30-156 constitute a C-type lectin-like domain, and amino acid 50 is a site for potential N-linked glycosylation. In addition, amino acids 127 and 142-143 form a ligand-binding surface. In isoform 2, there is a 79 aa substitution for the C-terminal 101 amino acids, while in isoform 3, there is an 11 aa substitution for the C-terminal 57 amino acids. Human Reg4 shares 68% and 67% aa sequence identity with rat and mouse Reg4, respectively. Like other members of the regenerating gene family, Reg4 is expressed in the gastrointestinal (GI) tract and ectopically at other sites in the setting of tissue injury (1, 2). In particular, Reg4 is expressed in normal colon mucosa, and up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, inflammatory bowel disease (Crohn’s disease and ulcerative colitis), and, outside the GI tract, in prostate adenocarcinoma (2-8). The physiological function of Reg4 is presently unknown, but it may be involved in inflammatory and metaplastic responses of the GI epithelium (3).

References

  1. Hartupee, J.C. et al. (2001) Biochim. Biophys. Acta 1518:287.
  2. Zhang, Y. et al. (2003) World J. Gastroenterol. 9:2635.
  3. Kamarainen, M. et al. (2003) Am. J. Pathol. 163:11.
  4. Zhang, Y. et al. (2003) Cancer Lett. 200:69.
  5. Violette, S. et al. (2003) Int. J. Cancer 103:185.
  6. Oue, N. et al. (2005) J. Pathol. 207:185.
  7. Zhennan, G. et al. (2005) Clin. Cancer Res. 2:2237
  8. Bishnupuri, K.S. et al. (2006) Gastroenterology 130:137.

Long Name

Regenerating Islet-derived 4

Alternate Names

GISP, RELP

Entrez Gene IDs

83998 (Human); 67709 (Mouse); 445583 (Rat)

Gene Symbol

REG4

UniProt

Q9BYZ8

Additional Reg4 Products

Product Documents for Human Reg4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Reg4 Antibody

For research use only

Citations for Human Reg4 Antibody

Customer Reviews for Human Reg4 Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human Reg4 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 10/04/2019
    We used this antibody for a sandwich ELISA format in combination with mAb (1379) and protein (1379-RG). This combination worked very well and detected human Reg 4 in serum and plasma samples.
    Human Reg4 Antibody AF1379
  • Human Reg4 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: induced pluripotent stem cells and Intestinal organoids
    Species: Human
    Verified Customer | Posted 12/15/2016
    For immunostaining to make unique populations of secretory progenitor cells in intestinal stem cell-derived organoid cultures.
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 23769888
    Species: Mouse
    Verified Customer | Posted 01/05/2015

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