Human Reg4 Antibody

Catalog # Availability Size / Price Qty
AF1379
AF1379-SP
Reg4 in Human Colon Cancer Tissue.
1 Image
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (2)

Human Reg4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Reg4 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse (rm) Reg4, rmReg3A, and recombinant rat (rr) Reg3A is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Reg4
Asp23-Pro158
Accession # Q9BYZ8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Reg4 (Catalog # 1379-RG)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Reg4 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Reg4 in Human Colon Cancer Tissue. Reg4 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Reg4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1379) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg4

Reg4 (regenerating islet-derived protein 4; also named Reg-like protein, RELP, and gastrointestinal secretory protein) is a 17 kDa, secreted glycoprotein belonging to the calcium (C-type) dependent lectin superfamily and the regenerating gene family (1-3). There are three potential isoforms for human Reg4. Isoform 1 is synthesized as a 158 amino acid (aa) precursor with a 22 aa signal sequence and a 136 aa mature chain. Amino acids 30-156 constitute a C-type lectin-like domain, and amino acid 50 is a site for potential N-linked glycosylation. In addition, amino acids 127 and 142-143 form a ligand-binding surface. In isoform 2, there is a 79 aa substitution for the C-terminal 101 amino acids, while in isoform 3, there is an 11 aa substitution for the C-terminal 57 amino acids. Human Reg4 shares 68% and 67% aa sequence identity with rat and mouse Reg4, respectively. Like other members of the regenerating gene family, Reg4 is expressed in the gastrointestinal (GI) tract and ectopically at other sites in the setting of tissue injury (1, 2). In particular, Reg4 is expressed in normal colon mucosa, and up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, inflammatory bowel disease (Crohn’s disease and ulcerative colitis), and, outside the GI tract, in prostate adenocarcinoma (2-8). The physiological function of Reg4 is presently unknown, but it may be involved in inflammatory and metaplastic responses of the GI epithelium (3).

References
  1. Hartupee, J.C. et al. (2001) Biochim. Biophys. Acta 1518:287.
  2. Zhang, Y. et al. (2003) World J. Gastroenterol. 9:2635.
  3. Kamarainen, M. et al. (2003) Am. J. Pathol. 163:11.
  4. Zhang, Y. et al. (2003) Cancer Lett. 200:69.
  5. Violette, S. et al. (2003) Int. J. Cancer 103:185.
  6. Oue, N. et al. (2005) J. Pathol. 207:185.
  7. Zhennan, G. et al. (2005) Clin. Cancer Res. 2:2237
  8. Bishnupuri, K.S. et al. (2006) Gastroenterology 130:137.
Long Name
Regenerating Islet-derived 4
Entrez Gene IDs
83998 (Human); 67709 (Mouse); 445583 (Rat)
Alternate Names
Gastrointestinal secretory protein; GISP; GISPREG-4; Reg IV; Reg4; regenerating gene type IV; regenerating islet-derived family, member 4; regenerating islet-derived protein 4; Regenerating islet-derived protein IV; REG-IV; RELP; RELPREG-like protein

Product Datasheets

You must select a language.

x

Citations for Human Reg4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Filter by:

  1. REG gene expression in inflamed and healthy colon mucosa explored by in situ hybridisation.
    Authors: van Beelen Granlund A, Ostvik AE, Brenna O et al.
    Cell Tissue Res
  2. REG4 Is Highly Expressed in Mucinous Ovarian Cancer: A Potential Novel Serum Biomarker
    Authors: Laura Lehtinen, Pia Vesterkvist, Pia Roering, Taina Korpela, Liisa Hattara, Katja Kaipio et al.
    PLOS ONE
  3. ARTS mediates apoptosis and regeneration of the intestinal stem cell niche
    Authors: E Koren, Y Yosefzon, R Ankawa, D Soteriou, A Jacob, A Nevelsky, R Ben-Yosef, G Bar-Sela, Y Fuchs
    Nat Commun, 2018-11-02;9(1):4582.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. IL2RG, identified as overexpressed by RNA-seq profiling of pancreatic intraepithelial neoplasia, mediates pancreatic cancer growth
    Authors: M Ayars, E O'Sullivan, A Macgregor-, K Shindo, H Kim, M Borges, J Yu, RH Hruban, M Goggins
    Oncotarget, 2017-08-03;8(48):83370-83383.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Reg4+ deep crypt secretory cells function as epithelial niche for Lgr5+ stem cells in colon
    Proc Natl Acad Sci USA, 2016-08-29;113(37):E5399-407.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  6. RNA sequencing of sessile serrated colon polyps identifies differentially expressed genes and immunohistochemical markers.
    Authors: Delker D, McGettigan B, Kanth P, Pop S, Neklason D, Bronner M, Burt R, Hagedorn C
    PLoS ONE, 2014-02-12;9(2):e88367.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. REG4 acts as a mitogenic, motility and pro-invasive factor for colon cancer cells.
    Authors: Rafa L, Dessein AF, Devisme L, Buob D, Truant S, Porchet N, Huet G, Buisine MP, Lesuffleur T
    Int. J. Oncol., 2010-03-01;36(3):689-98.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Reg IV is a serum biomarker for gastric cancer patients and predicts response to 5-fluorouracil-based chemotherapy.
    Authors: Mitani Y, Oue N, Matsumura S, Yoshida K, Noguchi T, Ito M, Tanaka S, Kuniyasu H, Kamata N, Yasui W
    Oncogene, 2007-01-22;26(30):4383-93.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Activation of REG family proteins in colitis.
    Authors: Granlund Av, Beisvag V, Torp SH et al.
    Scand J Gastroenterol

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Reg4 Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Reg4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Reg4 Antibody
By Anonymous on 10/04/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody for a sandwich ELISA format in combination with mAb (1379) and protein (1379-RG). This combination worked very well and detected human Reg 4 in serum and plasma samples.


Human Reg4 Antibody
By Anonymous on 12/15/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: induced pluripotent stem cells,Intestinal organoids Species: Human

For immunostaining to make unique populations of secretory progenitor cells in intestinal stem cell-derived organoid cultures.