Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Ret Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Ret
Leu29-Arg635
Accession # P07949

Specificity

Detects human Ret in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Ret is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Ret Antibody

Ret antibody in Human Spinal Cord by Immunohistochemistry (IHC-P).

Ret in Human Spinal Cord.

Ret was detected in immersion fixed paraffin-embedded sections of human spinal cord using Goat Anti-Human Ret Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1485) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to motor neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Ret Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spinal cord

Western Blot

0.1 µg/mL
Sample: Recombinant Human Ret (Catalog # 1168-CR)

Reviewed Applications

Read 5 reviews rated 5 using AF1485 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ret

The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha ‑1‑4). GFR alpha -1, -2, -3, and -4 are the preferred ligand-binding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (1-4).

References

  1. Trupp, M. et al. (1998) Mol. Cell. Neurosci. 11:47.
  2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
  3. Carlomagno, F. et al. (1998) Endocrinology 139:3613.
  4. Baloh, R. et al. (1998) Neuron 21:1291.

Long Name

Proto-oncogene Tyrosine-protein Kinase Receptor Ret

Alternate Names

CDHF12MTC1, CDHR16, EC 2.7.10, Hirschsprung disease 1, Hirschsprung disease), MEN2A, MEN2B, multiple endocrine neoplasia and medullary thyroid carcinoma 1, ret proto-oncogene, RET transforming sequence

Entrez Gene IDs

5979 (Human); 19713 (Mouse)

Gene Symbol

RET

UniProt

P07949

Additional Ret Products

Product Documents for Human Ret Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Ret Antibody

For research use only

Related Research Areas

Cancer Biomarkers
GDNF Family

Citations for Human Ret Antibody

Customer Reviews for Human Ret Antibody (5)

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5 Customer Ratings
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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Ret in neuroblastoma cells and of recombinant human Ret expressed in 293 cells and MG87 cells
    Species: Human
    Verified Customer | Posted 12/18/2014
    Immunofluorescence analysis of Ret in MG87 cells transfected with human Ret (green)
    Human Ret Antibody AF1485
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Endogenous Ret in neuroblastoma cells and of recombinant human Ret expressed in 293 cells and MG87 cells
    Species: Human
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Endogenous Ret in mouse sections
    Species: Mouse
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse tissue homogenates
    Species: Mouse
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: 293 cells and MG87 cells transfected with human Ret
    Species: Human
    Verified Customer | Posted 12/18/2014

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Protocols

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