Human RGM-B Antibody

Catalog # Availability Size / Price Qty
MAB3630
MAB3630-SP
Product Details
Citations (1)
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Human RGM-B Antibody Summary

Species Reactivity
Human
Specificity
Detects human RGM-B in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse RGM-B, recombinant human (rh) RGM-A, or rhRGM-C is observed.
Source
Monoclonal Mouse IgG2B Clone # 398528
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RGM-B isoform 1
Gly46-Ser411
Accession # Q6NW40
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human RGM-B (Catalog # 3630-RG) under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-B

RGM-B, also known as DRAGON, is a 40 kDa member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane proteins (1, 2). It is synthesized as a preproprotein that consists of a 45 amino acid (aa) signal sequence, a 368 aa mature region, and a 24 aa C-terminal prosegment (3). RGM-B contains an RGD motif, two potential N-linked glycosylation sites, and an abbreviated von Willebrand factor domain. There is a potential proteolytic cleavage site within the VWF domain (4). Alternative splicing may generate isoforms of RGM-B with N-terminal extensions or truncation following the VWF domain. Mature human RGM-B shares 52% and 36% aa sequence identity with the comparable regions of RGM-A and RGM-C, respectively. It shares 98%, 92%, 92%, and 78% aa sequence identity with macaque, mouse, bovine, and chicken RGM-B, respectively. RGM-B is expressed in the developing and adult nervous system, particularly in the dorsal root ganglia and mantle layer of the spinal cord (3‑5). In mouse, it shows a complementary, non-overlapping distribution with RGM-A (2‑5). RGM-B is also expressed in fetal and adult enteric ganglia and in postnatal intestinal epithelium (6). RGM-B expression has been detected in neuronal cell bodies and proximal axonal segments (4) but is also present on the cell surface, where it interacts homophilically and mediates neuronal adhesion (3). RGM-B additionally functions as a BMP coreceptor. It directly binds BMP-2 and -4 but not other TGF-beta family proteins (7). RGM-B associates with BMP type I (ALK-2, -3, -6) and type II (Activin RIIA, Activin RIIB) receptors and enhances BMP signaling (7).

References
  1. Monnier, P.P. et al. (2002) Nature 419:392.
  2. Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
  3. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  4. Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
  5. Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
  6. Metzger, M. et al. (2005) Dev. Dyn. 234:169.
  7. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
Long Name
Repulsive Guidance Molecule B
Entrez Gene IDs
285704 (Human); 68799 (Mouse)
Alternate Names
DKFZp434P228; Dragon; DRG11-responsive axonal guidance and outgrowth of neurite; RGM domain family, member B; RGMB; RGM-B

Product Datasheets

Citation for Human RGM-B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance.
    Authors: Xiao, Yanping, Yu, Sanhong, Zhu, Baogong, Bedoret, Denis, Bu, Xia, Francisco, Loise M, Hua, Ping, Duke-Cohan, Jonathan, Umetsu, Dale T, Sharpe, Arlene H, DeKruyff, Rosemari, Freeman, Gordon J
    J Exp Med, 2014;211(5):943-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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