Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2311A
View all RTTN Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human RTTN
Accession # Q86VV8

Specificity

Detects human RTTN in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human RTTN Antibody

Detection of Human RTTN antibody by Western Blot.

Detection of Human RTTN by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human RTTN Monoclonal Antibody (Catalog # MAB9966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RTTN at approximately 248 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RTTN antibody in HDLM-2 Human Cell Line by Immunocytochemistry (ICC).

RTTN in HDLM‑2 Human Cell Line.

RTTN was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Rabbit Anti-Human RTTN Monoclonal Antibody (Catalog # MAB9966) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
RTTN antibody in Human Stomach by Immunohistochemistry (IHC-P).

RTTN in Human Stomach.

RTTN was detected in immersion fixed paraffin-embedded sections of human stomach using Rabbit Anti-Human RTTN Monoclonal Antibody (Catalog # MAB9966) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human RTTN Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human RTTN Monoclonal Antibody(Catalog # MAB99661).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HDLM-2 human Hodgkin's lymphoma cell line

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 1 review rated 5 using MAB9966 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: RTTN

Human Rotatin, or RTTN, is a 2,226 amino acids (aa) protein discovered in a large-scale gene trap screen for factors that govern left-right specification. RTTN plays a role in the maintenance of a normal ciliary structure and is required for correct asymmetric expression of NODAL, LEFTY and PITX2.  In humans, RTTN mutations link aberrant ciliary function to abnormal development and organization of the cortex and might be a cause of Primary Microcephaly, Primordial Dwarfism and other brain malformations.

Long Name

Rotatin

Alternate Names

MSSP, PMGYS, Rotatin

Entrez Gene IDs

25914 (Human); 246102 (Mouse); 291377 (Rat)

Gene Symbol

RTTN

UniProt

Q86VV8

Additional RTTN Products

Product Documents for Human RTTN Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human RTTN Antibody

For research use only

Customer Reviews for Human RTTN Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human RTTN Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human RTTN Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Stomach tissue
    Species: Human
    Verified Customer | Posted 07/04/2022
    Human RTTN Antibody MAB9966

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies