Human RTTN Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of Human RTTN by Western Blot.
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Human RTTN Antibody Summary

Species Reactivity
Detects human RTTN in direct ELISAs.
Recombinant Monoclonal Rabbit IgG Clone # 2311A
Protein A or G purified from cell culture supernatant
Synthetic peptide containing human RTTN
Accession # Q86VV8
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human RTTN Monoclonal Antibody(Catalog # MAB99661).

This product is intended for assay development on various assay platforms requiring antibody pairs.

1-25 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human RTTN antibody by Western Blot. View Larger

Detection of Human RTTN by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human RTTN Monoclonal Antibody (Catalog # MAB9966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RTTN at approximately 248 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry RTTN antibody in HDLM-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

RTTN in HDLM‑2 Human Cell Line. RTTN was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Rabbit Anti-Human RTTN Monoclonal Antibody (Catalog # MAB9966) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry RTTN antibody in Human Stomach by Immunohistochemistry (IHC-P). View Larger

RTTN in Human Stomach. RTTN was detected in immersion fixed paraffin-embedded sections of human stomach using Rabbit Anti-Human RTTN Monoclonal Antibody (Catalog # MAB9966) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: RTTN

Human Rotatin, or RTTN, is a 2,226 amino acids (aa) protein discovered in a large-scale gene trap screen for factors that govern left-right specification. RTTN plays a role in the maintenance of a normal ciliary structure and is required for correct asymmetric expression of NODAL, LEFTY and PITX2.  In humans, RTTN mutations link aberrant ciliary function to abnormal development and organization of the cortex and might be a cause of Primary Microcephaly, Primordial Dwarfism and other brain malformations.

Long Name
Entrez Gene IDs
25914 (Human); 246102 (Mouse); 291377 (Rat)
Alternate Names
DKFZp434G145; FLJ26356; FLJ39085; MSSP; PMGYS; Rotatin; RTTN

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Human RTTN Antibody
By Anonymous on 07/04/2022
Application: IHC Sample Tested: Stomach tissue Species: Human