Human S100A4 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human S100A4 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 922813
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human S100A4
    Met1-Ser101
    Accession # P26447
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse S100A4 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # MAB4137) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100A4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
S100A4 in Human Cervical Cancer Tissue. S100A4 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # MAB4137) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunocytochemistry
S100A4 in A549 Human Cell Line. S100A4 was detected in immersion fixed A549 human lung carcinoma cells unstimulated (lower panel) and stimulated with the EMT Inducing Media Supplement (upper panel; Catalog # CCM017) using Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # MAB4137) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A4

S100A4, also known as Metastasin, Mtsl and Calvasculin, is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of proteins (1-5). The S100 family is further classified as a member of the EF-hand superfamily of Ca++-binding proteins. These participate in both calcium-dependent and
calcium-independent protein-protein interactions. The hallmark of this superfamily is the EF-hand motif that consists of a Ca++-binding site flanked by two alpha -helices (helix E and helix F) that were originally identified in a right-handed model of carp muscle calcium-binding protein (6). Human S100A4 is 101 amino acids (aa) in length (1, 2). It contains two EF hand domains, one between aa 12-47, and a second between aa 50-85. The first domain has a 14 aa cation-binding motif and binds Ca++ with low affinity. The second Ca++-binding motif is 12 aa in length and binds Ca++ with high affinity. S100A4 has no classical signal sequence but is secreted from cells (3, 7). Human S100A4 shares 93% and 95% aa identity with mouse and canine S100A4, respectively. S100A4 reportedly exists as a dimer (8, 9, 10). Extracellular S100A4 is reported to induce MMP production, activate MMPs, promote neurite outgrowth and stimulate cardiomyocyte proliferation (4, 10, 11, 12, 13). Within the cell, dimers are likely the functional unit. Here, they are constitutive homo- or heterodimers (with S100A1) that interact with Ca++, undergo a conformational change, and subsequently bind to cytoplasmic targets. Known targets include p53, myosin heavy chain II, F-actin and liprin beta 1 (4, 14). In general, it can be said that S100A4 blocks target phosphorylation and multimerization (4, 7, 14). S100A4 activity has been associated with cell transformation. It seems likely this is either coincidental, or a consequence, rather than a cause of transformation (3).

  • References:
    1. Engelkamp, D. et al. (1992) Biochemistry 31:10258.
    2. Tomida, Y. et al. (1992) Biochem. Biophys. Res. Commun. 189:1310.
    3. Garrett, S.C. et al. (2006) J. Biol. Chem. 281:677.
    4. Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.
    5. Donato, R. (2001) Int. J. Biochem. Mol. Biol. 33:637.
    6. Kretsinger, R.H. and C.E. Nockolds (1973) J. Biol. Chem. 248:3313.
    7. Helfman, D.M. et al. (2005) Br. J. Cancer 92:1955.
    8. Burkitt, W.I. et al. (2003) Biochem. Soc. Trans. 31:985.
    9. Vallaly, K.M. et al. (2002) Biochemistry 41:12670.
    10. Novitskaya, V. et al. (2000) J. Biol. Chem. 275:41278.
    11. Stary, M. et al. (2006) Biochem. Biophys. Res. Commun. 343:555.
    12. Semov, A. et al. (2005) J. Biol. Chem. 280:20833.
    13. Saleem, M. et al. (2006) Proc. Natl. Acad. Sci. USA 103:14825.
    14. Kriajevska, M. et al. (2002) J. Biol. Chem. 277:5229.
  • Long Name:
    S100 Calcium Binding Protein A4
  • Entrez Gene IDs:
    6275 (Human); 20198 (Mouse); 24615 (Rat)
  • Alternate Names:
    18A2; 42A; Calvasculin; CAPL; CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin; fibroblast-specific protein-1,42A; FSP1; leukemia multidrug resistance associated protein; malignant transformation suppression 1; Metastasin; MTS1; murine placental homolog); P9KA; PEL98; Placental calcium-binding protein; Protein Mts1; protein S100-A4; S100 calcium binding protein A4; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog); S100 calcium-binding protein A4; S100A4
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