Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all S100B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100B
Met1-Glu92
Accession # P04271

Specificity

Detects human S100B in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) S100A1, rhS100A4, and rhS100A6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human S100B Antibody

S100B antibody in Human Alzheimer's Brain by Immunohistochemistry (IHC-P).

S100B in Human Alzheimer'S Brain.

S100B was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Goat Anti-Human S100B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1820) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human S100B antibody by Simple WesternTM.

Detection of Human S100B by Simple WesternTM.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for S100B at approximately 4 kDa (as indicated) using 10 µg/mL of Goat Anti-Human S100B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1820). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human S100B Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain

Simple Western

10 µg/mL
Sample: Human brain (cerebellum) tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human S100B

Reviewed Applications

Read 1 review rated 5 using AF1820 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100B

S100B belongs to the S100 subgroup of the EF-hand family of Ca2+ binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Long Name

S100 Calcium Binding Protein B

Alternate Names

beta (neural), NEF, S100, S100 beta, S100 calcium binding protein B, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural), S-100 calcium-binding protein, beta chain, 10protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100beta

Entrez Gene IDs

6285 (Human)

Gene Symbol

S100B

UniProt

P04271

Additional S100B Products

Product Documents for Human S100B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S100B Antibody

For research use only

Citations for Human S100B Antibody

Customer Reviews for Human S100B Antibody (1)

5 out of 5
1 Customer Rating
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  • Human S100B Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: mouse skin tissue
    Species: Mouse
    Verified Customer | Posted 08/18/2017
    Tissue: formalin fixed paraffin embedded mouse skin tissue
    1.Tissue: formalin fixed paraffin embedded mouse skin tissue 2.Antigen retrieval: 0.01 M Citric Acid, 0.05% Tween-20 3.First antibody: Goat anti s100b (AF1820), 1:100 dilution overnight at 4 °C 4.Secondary antibody- Bio- Dondey anti Goat, 1:200 room temperature 1 hours 5.Kits used : Vectorstain ABC kit (cat: PK-4000) and DAB staining kit (Brown, cat: SK4100) 6.Counterstained with hematoxylin (blue), only nerve and hair follicle are positive.
    Human S100B Antibody AF1820

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