Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1012512
View all S1P4/EDG-6 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human S1P4/EDG‑6
Met1-Ile384
Accession # O95977

Specificity

Detects human S1P

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human S1P4/EDG‑6 Antibody

Detection of S1P4/EDG-6 antibody in HEK293 Human Cell Line Transfected with Human S1P4/EDG-6 and eGFP antibody by Flow Cytometry.

Detection of S1P4/EDG-6 in HEK293 Human Cell Line Transfected with Human S1P4/EDG-6 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human S1P4/EDG-6 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human S1P4/EDG-6 Monoclonal Antibody (Catalog # MAB10321) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Applications for Human S1P4/EDG‑6 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human S1P4/EDG-6 and eGFP

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S1P4/EDG-6

EDG-6 belongs to the G protein-coupled receptor 1 family. It is specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. EDG-6 is a receptor for the lysosphingolipid sphingosine 1- phosphate (S1P), a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. EDG-6 may be involved in cell migration processes that are specific for lymphocytes.

Long Name

Endothelial Differentiation Gene 5

Alternate Names

EDG6, S1PR4, SLP4

Entrez Gene IDs

8698 (Human); 13611 (Mouse); 314649 (Rat)

Gene Symbol

S1PR4

UniProt

O95977

Additional S1P4/EDG-6 Products

Product Documents for Human S1P4/EDG‑6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S1P4/EDG‑6 Antibody

For research use only

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