Human S1P4/EDG-6 Antibody Summary
Accession # O95977
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of S1P4/EDG-6 in HEK293 Human Cell Line Transfected with Human S1P4/EDG-6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human S1P4/EDG-6 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human S1P4/EDG-6 Monoclonal Antibody (Catalog # MAB10321) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
EDG-6 belongs to the G protein-coupled receptor 1 family. It is specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. EDG-6 is a receptor for the lysosphingolipid sphingosine 1- phosphate (S1P), a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. EDG-6 may be involved in cell migration processes that are specific for lymphocytes.
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