Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 776807
View all S1P3/EDG-3 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human S1P3/EDG‑3
Accession # Q99500

Specificity

Detects human S1P3/EDG‑3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human S1P3/EDG-3 Antibody

S1P3/EDG-3 antibody in Human Spleen by Immunohistochemistry (IHC-P).

S1P3/EDG‑3 in Human Spleen.

S1P3/EDG-3 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human S1P3/EDG-3 Monoclonal Antibody (Catalog # MAB75241) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the marginal zone of germinal centers. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human S1P3/EDG-3 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S1P3/EDG-3

EDG-3, also known as sphingosine 1-phosphate receptor-3 (S1P3), is a 378 amino acid (aa) seven-transmembrane receptor for the lysolipid phosphoric acid mediator, sphingosine 1-phosphate (S1P). EDG-3 interactions with S1P induce a range of responses including vasoconstriction, brachycardia, vascular endothelial and smooth muscle cell proliferation and migration, heart and liver fibrosis, and coagulation-induced inflammation. Extracellular portions of human EDG-3 show 74% aa identity with mouse and rat EDG-3.

Long Name

Endothelial Differentiation Gene 3

Alternate Names

EDG3, LPB3, S1PR3

Entrez Gene IDs

1903 (Human); 13610 (Mouse); 306792 (Rat)

Gene Symbol

S1PR3

UniProt

Q99500 (Human)

Additional S1P3/EDG-3 Products

Product Documents for Human S1P3/EDG-3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S1P3/EDG-3 Antibody

For research use only

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Protocols

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