S1P3/EDG‑3 in Human Spleen.
S1P3/EDG‑3 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human S1P3/EDG‑3 Monoclonal Antibody (Catalog # MAB75241) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the marginal zone of germinal centers. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
EDG-3, also known as sphingosine 1-phosphate
receptor-3 (S1P3), is a 378 amino acid (aa) seven-transmembrane receptor for
the lysolipid phosphoric acid mediator, sphingosine 1-phosphate (S1P). EDG-3
interactions with S1P induce a range of responses including vasoconstriction,
brachycardia, vascular endothelial and smooth muscle cell proliferation and
migration, heart and liver fibrosis, and coagulation-induced inflammation. Extracellular
portions of human EDG-3 show 74% aa identity with mouse and rat EDG-3.
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