Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 282503
View all S1P5/EDG-8 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human S1P5/EDG‑8
Met1-Asp398
Accession # Q9H228

Specificity

Detects human S1P

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human S1P5/EDG‑8 Antibody

Detection of S1P5/EDG-8 antibody in HEK293 Human Cell Line Transfected with Human S1P5/EDG-8 and eGFP antibody by Flow Cytometry.

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG‑8 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human S1P5/EDG-8 and eGFP were stained with either (A) Mouse Anti-Human S1P5/EDG-8 Monoclonal Antibody (Catalog # MAB3964) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Applications for Human S1P5/EDG‑8 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human S1P5/EDG‑8 and eGFP

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S1P5/EDG-8

S1P5 is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P5 is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P5 show 96% and 97% aa identity with mouse and rat S1P5, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P5 is upregulated in large granular lymphocytic leukemias.

Long Name

Endothelial Differentiation Gene 8

Alternate Names

EDG8, LPB4, S1PR5, SPPR-1, SPPR-2

Entrez Gene IDs

53637 (Human); 94226 (Mouse); 60399 (Rat)

Gene Symbol

S1PR5

UniProt

Q9H228

Additional S1P5/EDG-8 Products

Product Documents for Human S1P5/EDG‑8 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S1P5/EDG‑8 Antibody

For research use only

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