Detection of Human Serpin A11 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin A11 Monoclonal Antibody (Catalog # MAB8236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin A11 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A11
Serpin A11 (serine proteinase inhibitor – clade A#11) is a 50-60 kDa secreted member of the serpin superfamily of protease inhibitors. Serpins are the largest family of endogenous protease inhibitors, and target a wide variety of serine and cysteine proteases. In general, members of this superfamily regulate multiple proteolytic cascades, and are particularly effective due to the fact that their inhibitory activities can be fine-tuned through the participation of discrete, non-serpin co-factors. Serpin A11 gene expression is extremely high in normal liver, with lower levels detected in normal breast, breast tumor, and benign prostatic hyperplasia. Human Serpin A11 is 422 amino acids (aa) in length, with four potential N-linked glycosylation sites, and shares 75% aa sequence identity with mouse Serpin A11.
Serpin Peptidase Inhibitor, Clade A, Member 11
Entrez Gene IDs:
256394 (Human); 380780 (Mouse); 362774 (Rat)
Antiproteinase-Like 2; antitrypsin), member 11; member 11; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A11; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
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