Human Serpin A11 Antibody

Catalog # Availability Size / Price Qty
MAB8236
MAB8236-SP
Detection of Human Serpin A11 by Western Blot.
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Human Serpin A11 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SerpinA11 in ELISA and Western Blot.
Source
Monoclonal Mouse IgG2B Clone # 895842
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SerpinA11
Met1-Gly422
Accession # Q86U17
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Complete Your Experiment

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Serpin A11 by Western Blot. View Larger

Detection of Human Serpin A11 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin A11 Monoclonal Antibody (Catalog # MAB8236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin A11 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A11

Serpin A11 (serine proteinase inhibitor – clade A#11) is a 50-60 kDa secreted member of the serpin superfamily of protease inhibitors.  Serpins are the largest family of endogenous protease inhibitors, and target a wide variety of serine and cysteine proteases.  In general, members of this superfamily regulate multiple proteolytic cascades, and are particularly effective due to the fact that their inhibitory activities can be fine-tuned through the participation of discrete, non-serpin co-factors.  Serpin A11 gene expression is extremely high in normal liver, with lower levels detected in normal breast, breast tumor, and benign prostatic hyperplasia.  Human Serpin A11 is 422 amino acids (aa) in length, with four potential N-linked glycosylation sites, and shares 75% aa sequence identity with mouse Serpin A11.

Long Name
Serpin Peptidase Inhibitor, Clade A, Member 11
Entrez Gene IDs
256394 (Human); 380780 (Mouse); 362774 (Rat)
Alternate Names
Antiproteinase-Like 2; antitrypsin), member 11; member 11; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A11; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

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Secondary Antibodies

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