Human Serpin A9/Centerin Antibody

Catalog # Availability Size / Price Qty
MAB5429
MAB5429-SP
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Human Serpin A9/Centerin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin A9/Centerin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Serpin A1, A3, A4, A5, A6, A11, A12, B6, B8, B9, C1, D1, E1, E2, F1, F2, G1, I1, I2, recombinant mouse (rm) SerpinA3N, and rmSerpinA9 is observed. 
Source
Monoclonal Mouse IgG2A Clone # 585302
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A9/Centerin
Ala24-Thr417
Accession # AAQ89063
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
Immersion-fixed paraffin-embedded sections of human B cell lymphoma

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A9/Centerin

Serpin A9 (Serine proteinase inhibitor-clade A #9; also GCET1, Centerin and Serpin A11) is a 46 kDa, secreted member of the alpha 1-antitrypsin (clade A) subfamily, serpin superfamily of protease inhibitors. It is only produced by normal and lymphoma germinal center B cells. Its function is unknown, but it presumably acts as an inhibitor of a trypsin-like protease. Mature human Serpin A9 is presumably 394 amino acids (aa) in length. It contains one Serpin motif (aa 405-415). There are multiple splice variants associated with Serpin A9. There are alternate start sites at Met19, Met99 and Met150. A fourth isoform shows a two aa substitution for aa 369-435, while a fifth isoform shares this same substitution coupled with a 39 aa substitution for aa 1-75. A sixth variant shows a deletion of aa 91-190. Mature human Serpin A9 shares 67% aa identity with mouse Serpin A9.

Long Name
Serpin Peptidase Inhibitor, Clade A, Member 9
Entrez Gene IDs
327657 (Human); 71907 (Mouse); 299274 (Rat)
Alternate Names
Centerin; GCET1; Serpin A9; SERPINA11; SERPINA11b

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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