Human Serpin B8/Proteinase Inhibitor 8 Antibody

Catalog # Availability Size / Price Qty
MAB4158
MAB4158-SP
Serpin B8/Proteinase Inhibitor 8 in Human Pancreas.
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Human Serpin B8/Proteinase Inhibitor 8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin B8/Proteinase Inhibitor 8 in direct ELISAs. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) Serpin B8 is observed. No cross-reactivity with recombinant human Serpin A1, A3, A4, A5, A6, A9, A11, A12, B3, B6, B9, C1, D1, E1, E2, F1, F2, G1, I1, I2, rmSerpin A1, A3N, A6, A9, C1, D1, E1, E2, F1, F2, I1, recombinant rat (rr) Serpin A6, or rrSerpin A12 is observed.
Source
Monoclonal Mouse IgG2A Clone # 423023
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf21-derived recombinant human Serpin B8/Proteinase Inhibitor 8
Asp2-Pro374
Accession # P50452
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Serpin B8/Proteinase Inhibitor 8 in Human Pancreas. View Larger

Serpin B8/Proteinase Inhibitor 8 in Human Pancreas. Serpin B8/Proteinase Inhibitor 8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Human Serpin B8 Monoclonal Antibody (Catalog # MAB4158) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin B8/Proteinase Inhibitor 8

Serpin B8 (serine proteinase inhibitor-clade B #8; also PI8) is a 42‑45 kDa, cytoplasmic and secreted member of the ovalbumin (clade B)-subfamily, Serpin superfamily of protease inhibitors. It is produced by multiple cell types and shows inhibitory activity towards furin and other proteinases such as chymotrypsin. Human Serpin B8 is 374 amino acids (aa) in length. Although it has no signal sequence, it is released by platelets. The active inhibitor site lies between Arg336 and Arg342. One potential isoform shows a 2 aa substitution for the C-terminal 134 amino acids. Full length human Serpin B8 shares 78% and 83% aa identity with mouse and canine Serpin B8, respectively.

Entrez Gene IDs
5271 (Human); 20725 (Mouse); 288937 (Rat)
Alternate Names
CAP2; CAP-2; CAP2clade B (ovalbumin), member 8; Cytoplasmic antiproteinase 2; NK10; Peptidase inhibitor 8; PI8; PI-8; protease inhibitor 8 (ovalbumin type); Serpin B8; serpin peptidase inhibitor, clade B (ovalbumin), member 8

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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