Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 902728
View all Serum Amyloid A1/A2 Primary Antibodies »

Product Specifications

Immunogen

Human SAA1 synthetic peptide
Accession # P0DJI8

Specificity

Detects human SAA1/SAA2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Serum Amyloid A1/A2 Antibody

Detection of Human Serum Amyloid A1/A2 antibody by Western Blot.

Detection of Human Serum Amyloid A1/A2 by Western Blot.

Western blot shows lysates of human spleen tissue, human serum, and human plasma. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30192) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Recombinant Human Serum Amyloid A1/A2 antibody by Western Blot.

Detection of Recombinant Human Serum Amyloid A1/A2 by Western Blot.

Western blot shows 5 ng of Recombinant Human SAA1 and Recombinant Human SAA2. PVDF Membrane was probed with 0.2 µg/mL of Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30192) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Serum Amyloid A1/A2 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: Human spleen tissue, human serum, human plasma, recombinant human SAA1, and recombinant human SAA2

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serum Amyloid A1/A2

Human SAA1 is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 104 amino acid (aa), non‑glycosylated polypeptide that displaces apoA1 in the HDL3 complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only seven amino acids. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA1 shares 72%, 82%, and 72% aa sequence identity with mature mouse, rabbit, and hamster SAA1, respectively.

Alternate Names

PIG4;SAA;SAA1;SAA2;Serum amyloid A-1 protein;Serum amyloid A-2 protein;TP53I4

UniProt

P0DJI8

Additional Serum Amyloid A1/A2 Products

Product Documents for Human Serum Amyloid A1/A2 Antibody

Certificate of Analysis

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Product Specific Notices for Human Serum Amyloid A1/A2 Antibody

For research use only

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