Human sFRP-1 Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human sFRP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse sFRP‑2, recombinant human (rh) sFRP-3, and rhsFRP-4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human sFRP-1 (R&D Systems, Catalog # 1384-SF) 
    Ser32-Lys314
    Accession # AAB70793
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human sFRP‑1 (Histidine-tagged) (Catalog # 1384-SF)
  • Immunohistochemistry
    1-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
sFRP‑1 in Human Kidney. sFRP‑1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human sFRP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1384) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunocytochemistry
sFRP‑1 in MBA‑MB‑468 Human Cell Line. sFRP‑1 was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Goat Anti-Human sFRP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1384) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: sFRP-1

Secreted Frizzled Related Proteins (sFRPs) are a family of secreted, soluble vertebrate glycoproteins which contain homology to the Wnt-binding domain of the Frizzled (Fz) family of transmembrane receptors. sFRPs are approximately 30-35 kDa in size and are comprised of 3 domains: a signal sequence; an N-terminal Fz cysteine-rich domain (CRD) with 10 conserved cysteines; and a C-terminal heparin-binding region with weak homology to Netrin. The Fz CRD mediates Wnt-binding and is present in all Fz and sFRP family members (1).

sFRP-1, also known as secreted apoptosis-related protein 2 (SARP-2), FRP and FrzA, is expressed in the embryonic kidney, eye, brain, teeth, salivary gland and small intestine, most often at sites of epithelial-mesenchyme interaction (5). Expression in the adult animal is strong in the eye, kidney, and heart and also prevalent in the brain and lung (2, 5). sFRP-1 was first characterized as a protein that enhances the sensitivity of cells to apoptotic stimuli (3) and as an antagonist of Wnt signaling in Xenopus embryos (4). It is also characterized as a tumor suppressor in breast (6) and cervical carcinomas (7). In contrast, sFRP-1 is expressed by the majority of malignant gliomas (8) and contributes to the development of uterine leiomyomas (9), suggesting that the role of sFRP-1 is dependent on cell context. sFRP-1 has diverse activities, from inducing angiogenesis (10) in a variety of in vivo models to helping regulate Wnt-4 signaling (with sFRP-2) in renal organogenesis (11). Mouse and human sFRP-1 proteins share 94% amino acid identity (1).

  • References:
    1. Jones, S. et al. (2002) Bioessays 24:811.
    2. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2859.
    3. Melkonyan, H. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13636.
    4. Finch, P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6770.
    5. Leimeister, C. et al. (1998) Mech. Dev. 75:29.
    6. Ugolini, F. et al. (2001) Oncogene 20:5810.
    7. Ko, J. et al. (2002) Exp. Cell. Res. 280:280.
    8. Roth, W. et al. (2000) Oncogene 19:4210.
    9. Fukuhara, K, et al. (2002) J. Clin. Endocr. Metab. 87:1729.
    10. Dufourcq, P. et al. (2002) Circulation 106: 3097.
    11. Yoshino, K. et al. (2001) Mech. Dev. 102:45.
  • Long Name:
    Secreted Frizzled Related Protein 1
  • Entrez Gene IDs:
    6422 (Human); 20377 (Mouse); 84402 (Rat)
  • Alternate Names:
    FRP1; FRP-1FrzA; FRPSARP-2; FrzA; SARP-2; SARP2sFRP-1; Secreted apoptosis-related protein 2; secreted frizzled-related protein 1; sFRP1; sFRP-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Low expression of RBMS3 and SFRP1 are associated with poor prognosis in patients with gastric cancer
    Am J Cancer Res, 2016;6(11):2679-2689.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  2. Blockade of Wnt signaling inhibits angiogenesis and tumor growth in hepatocellular carcinoma.
    Authors: Hu J, Dong A, Fernandez-Ruiz V, Shan J, Kawa M, Martinez-Anso E, Prieto J, Qian C
    Cancer Res., 2009;69(17):6951-9.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  3. Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
    Authors: Gogolla N, Galimberti I, Deguchi Y, Caroni P
    Neuron, 2009;62(4):510-25.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Wnt signaling antagonists are potential prognostic biomarkers for the progression of radiographic hip osteoarthritis in elderly Caucasian women.
    Authors: Lane NE, Nevitt MC, Lui LY, de Leon P, Corr M
    Arthritis Rheum., 2007;56(10):3319-25.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  5. Genomics identifies medulloblastoma subgroups that are enriched for specific genetic alterations.
    Authors: Thompson MC, Fuller C, Hogg TL, Dalton J, Finkelstein D, Lau CC, Chintagumpala M, Adesina A, Ashley DM, Kellie SJ, Taylor MD, Curran T, Gajjar A, Gilbertson RJ
    J. Clin. Oncol., 2006;24(12):1924-31.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
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